Innovative use of platinum compounds to selectively detect live microorganisms by polymerase chain reaction

Takashi Soejima; Jun‐ichi Minami; Jin‐zhong Xiao; Fumiaki Abe

doi:10.1002/bit.25711

journal article Aug 19, 2015

Innovative use of platinum compounds to selectively detect live microorganisms by polymerase chain reaction

Takashi Soejima Jun‐ichi Minami Jin‐zhong Xiao Fumiaki Abe

Biotechnology and Bioengineering Vol. 113 No. 2 pp. 301-310 · Wiley

View at Publisher Save 10.1002/bit.25711

Abstract

ABSTRACTPCR cannot distinguish live microorganisms from dead ones. To circumvent this disadvantage, ethidium/propidium‐monoazide (EMA/PMA) and psoralen to discriminate live from dead bacteria have been used for 2 decades. These methods require the use of numerous laborious procedures. We introduce an innovative method that uses platinum compounds, which are primarily used as catalysts in organic chemistry and partly used as anti‐cancer drugs. Microorganisms are briefly exposed to platinum compounds in vivo, and these compounds penetrate dead (compromised) microorganisms but not live ones and are chelated by chromosomal DNA. The use of platinum compounds permits clear discrimination between live and dead microorganisms in water and milk (including Cronobacter sakazakii and Escherichia coli) via PCR compared with typically used PMA. This platinum‐PCR method could enable the specific detection of viable coliforms in milk at a concentration of 5–10 CFU mL−1 specified by EU/USA regulations after a 4‐h process. For sample components, environmental water contains lower levels of PCR inhibitors than milk does, and milk is similar to infant formula, skim milk and blood; thus, the use of the platinum‐PCR method could also prevent food poisoning due to the presence of C. sakazakii in dairy products. This method could provide outstanding rapidity for use in environmental/food/clinical tests. Platinum‐PCR could also be a substitute for the typical culture‐based methods currently used. Biotechnol. Bioeng. 2016;113: 301–310. © 2015 Wiley Periodicals, Inc.

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References

34

[1]

10.1111/j.1472-765x.1996.tb01131.x

[2]

10.1128/aem.00604-08

[3]

Chu G "Cellular responses to cisplatin. The roles of DNA‐binding proteins and DNA repair" J Biol Chem (1994) 10.1016/s0021-9258(17)42175-2

[4]

10.1093/nar/19.1.99

[5]

10.1128/jcm.36.7.1964-1968.1998

[6]

10.3201/eid1512.090252

[7]

10.1016/s0021-9258(20)80702-9

[8]

10.1038/nature08260

[9]

10.1016/s0021-9258(19)38277-8

[10]

10.1126/science.3175622

[11]

10.1016/0006-291x(85)90485-1

[12]

10.1073/pnas.0803441105

[13]

10.1111/j.1469-0691.2010.03453.x

[14]

McLaughlin F "A brief comparison of United States and European Union Standards for fluid dairy production" A Term Paper for ANR (Michigan State University) (2006)

[15]

10.4315/0362-028x.jfp-12-015

[16]

10.1111/j.1365-2672.2010.04716.x

[17]

10.1038/nmeth1007

[18]

10.4315/0362-028x-66.10.1798

[19]

10.1016/j.mimet.2006.04.015

[20]

10.1128/aem.01198-07

[21]

10.1128/jcm.00622-13

[22]

10.1038/205698a0

[23]

10.1038/222385a0

[24]

10.1128/aem.71.2.1018-1024.2005

[25]

10.1186/1471-2407-11-296

[26]

10.1128/aem.64.4.1313-1318.1998

[27]

10.1111/j.1348-0421.2007.tb03966.x

[28]

10.1128/jcm.02171-07

[29]

10.1007/s00253-012-4086-0

[30]

SoejimaT Schlitt‐DittrichF.2011. Method and kit for detection of microorganism. JPatent JP4825313 (B2)‐2011‐11‐30. (http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20111130&DB=EPODOC&locale=jp_EP&CC=JP&NR=4825313B2&KC=B2&ND=7).

[31]

SoejimaT Schlitt‐DittrichF.2013. Method and kit for detection of microorganism. CNY AU patent AU2010275576 (B2)‐2013‐02‐28. (http://worldwide.espacenet.com/publicationDetails/originalDocument?FT=D&date=20130228&DB=EPODOC&locale=jp_EP&CC=AU&NR=2010275576B2&KC=B2&ND=8).

[32]

10.1016/j.ab.2011.06.027

[33]

10.1128/aem.02895-10

[34]

Vaitilingom M "Direct detection of viable bacteria, molds and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment" Appl Environ Microbiol (1998) 10.1128/aem.64.3.1157-1160.1998

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Details

Published: Aug 19, 2015
Vol/Issue: 113(2)
Pages: 301-310
License: View

Authors

T

Takashi Soejima

Functional Food Ingredients Department Food Ingredients & Technology Institute

J

Jun‐ichi Minami

Functional Food Ingredients Department Food Ingredients & Technology Institute

J

Jin‐zhong Xiao

Next Generation Science Institute Morinaga Milk Industry Co., Ltd. 5‐1‐83, Higashihara Zama City Kanagawa Pref. 252‐8583 Japan

F

Fumiaki Abe

Functional Food Ingredients Department Food Ingredients & Technology Institute

Cite This Article

Takashi Soejima, Jun‐ichi Minami, Jin‐zhong Xiao, et al. (2015). Innovative use of platinum compounds to selectively detect live microorganisms by polymerase chain reaction. Biotechnology and Bioengineering, 113(2), 301-310. https://doi.org/10.1002/bit.25711

Innovative use of platinum compounds to selectively detect live microorganisms by polymerase chain reaction

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