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journal article Dec 20, 1995

A metabolic network stoichiometry analysis of microbial growth and product formation

W. M. van Gulik J. J. Heijnen
Biotechnology and Bioengineering Vol. 48 No. 6 pp. 681-698 · Wiley
View at Publisher Save 10.1002/bit.260480617
Abstract
AbstractUsing available biochemical information, metabolic networks have been constructed to describe the biochemistry of growth of Saccharomyces cerevisiae and Candida utilis on a wide variety of carbon substrates. All networks contained only two fitted parameters, the P/O ratio and a maintenance coefficient. It is shown that with a growth‐associated maintenance coefficient, K, of 1.37 mol ATP/ C‐mol protein for both yeasts and P/O ratios of 1.20 and 1.53 for S. cerevisiae and C. utilis, respectively, measured biomass yields could be described accurately. A metabolic flux analysis of aerobic growth of S. cerevisiae on glucose/ethanol mixtures predicted five different metabolic flux regimes upon transition from 100% glucose to 100% ethanol. The metabolic network constructed for growth of S. cerevisiae on glucose was applied to perform a theoretical exercise on the overproduction of amino acids. It is shown that theoretical operational product yield values can be substantially lower than calculated maximum product yields. A practical case of lysine production was analyzed with respect to theoretical bottlenecks limiting product formation. Predictions of network‐derived irreversibility limits for Ysp (μ) functions were compared with literature data. The comparisons show that in real systems such irreversibility constraints may be of relevance. It is concluded that analysis of metabolic network stoichiometry is a useful tool to detect metabolic limits and to guide process intensification studies. © 1995 John Wiley & Sons, Inc.
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References
37
[2]
Cássio F. "Transport of lactate and other short‐chain monocarboxylates in the yeast Saccharomyces cerevisiae" Appl. Environ: Microbiol. (1987) 10.1128/aem.53.3.509-513.1987
[3]
Cooper T. G. (1982)
[4]
de Jong‐Gubbels P. Vanrolleghem P. Heijnen J. J. van Dijken J. P. Pronk J. T.Regulation of carbon metabolism in chemostat cultures ofSaccharomyces cerevisiaegrown on mixtures of glucose and ethanol.Yeast(in press).
[5]
Eddy A. A. "Mechanisms of solute transport in selected eukaryotic micro‐organisms" Adv. Microbial Physiol. (1982)
[17]
Oura E.1972. The effect of aeration on the growth energetics and biochemical composition of baker's yeast Ph.D. thesis University of Helsinki Finland.
[18]
Roon R. J. "Negative interactions between amino acid and methylamine/ammonia transport systems of Saccharomyces cerevisiae" J. Biol. Chem. (1977) 10.1016/s0021-9258(17)40294-8
[19]
Russell J. B. "Energetics of bacterial growth, balance of anabolic and catabolic reactions" Microbiol. Rev. (1995) 10.1128/mr.59.1.48-62.1995
[25]
Serrano R. (1991)
[26]
Stouthamer A. H. "The search for correlation between theoretical and experimental growth yields" Microbial Biochem. (1979)
[28]
Vallino J. J. (1987)
[29]
Vallino J. J. Stephanopoulos G.1990.Intracellular flux analysis as means. of identifying limiting nodes in amino acid fermentations pp.1063–1066. In: C. Christiansen L. Munck and J. Villadsen (eds.) Proceedings of the 5th European Congress on Biotechnology. Munksgaard. Copenhagen.
[35]
Verduyn C. "A theoretical evaluation of growth yields of yeasts. Ant. van Leeuwenhoek Int" J. Gen. Mol. Microbiol. (1991)
[36]
Verduyn C. "Physiology of yeasts in relation to growth yields. Ant. van Leeuwenhoek" Int. J. Gen. Mol. Microbiol. (Special issue: Growth and Metabolism of Microorganisms) (1991)
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Metrics
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Citations
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References
Details
Published
Dec 20, 1995
Vol/Issue
48(6)
Pages
681-698
License
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Authors
W
W. M. van Gulik
J
J. J. Heijnen
Cite This Article
W. M. van Gulik, J. J. Heijnen (1995). A metabolic network stoichiometry analysis of microbial growth and product formation. Biotechnology and Bioengineering, 48(6), 681-698. https://doi.org/10.1002/bit.260480617
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