A microfluidic lung‐on‐a‐chip based on biomimetic hydrogel membrane

Chong Shen; Huiming Yang; Wenqi She; Qin Meng

doi:10.1002/bit.28426

journal article May 17, 2023

A microfluidic lung‐on‐a‐chip based on biomimetic hydrogel membrane

Chong Shen

Huiming Yang

Wenqi She Qin Meng

Biotechnology and Bioengineering Vol. 120 No. 7 pp. 2027-2038 · Wiley

View at Publisher Save 10.1002/bit.28426

Abstract

AbstractLung‐on‐chips have showed great promise as a tool to recapitulate the respiratory system for investigation of lung diseases in the past decade. However, the commonly applied artificial elastic membrane (e.g., polydimethylsiloxane, PDMS) in the chip failed to mimic the alveolar basal membrane in the composition and mechanical properties. Here we replaced the PDMS film by a thin, biocompatible, soft, and stretchable membrane based on F127‐DA hydrogel that well approached to the composition and stiffness of extracellular matrix in human alveoli for construction of lung‐on‐a‐chip. This chip well reconstructed the mechanical microenvironments in alveoli so that the epithelial/endothelial functions were highly expressed with a well established alveolar‐capillary barrier. In opposite to the unexpectedly accelerated fibrotic process on the PDMS‐based lung‐on‐a‐chip, HPAEpiCs on hydrogel‐based chip only presented fibrosis under nonphysiologically high strain, well reflecting the features of pulmonary fibrosis in vivo. This physiologically relevant lung‐on‐a‐chip would be an ideal model in investigation of lung diseases and for development of antifibrosis drugs.

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Metrics

25

Citations

50

References

Details

Published: May 17, 2023
Vol/Issue: 120(7)
Pages: 2027-2038
License: View

Authors

C

Chong Shen

Key Laboratory of Biomass Chemical Engineering (Education Ministry), College of Chemical and Biological Engineering Zhejiang University Hangzhou Zhejiang China

H

Huiming Yang

Key Laboratory of Biomass Chemical Engineering (Education Ministry), College of Chemical and Biological Engineering Zhejiang University Hangzhou Zhejiang China

W

Wenqi She

Key Laboratory of Biomass Chemical Engineering (Education Ministry), College of Chemical and Biological Engineering Zhejiang University Hangzhou Zhejiang China

Q

Qin Meng

Key Laboratory of Biomass Chemical Engineering (Education Ministry), College of Chemical and Biological Engineering Zhejiang University Hangzhou Zhejiang China

Funding

National Natural Science Foundation of China

Cite This Article

Chong Shen, Huiming Yang, Wenqi She, et al. (2023). A microfluidic lung‐on‐a‐chip based on biomimetic hydrogel membrane. Biotechnology and Bioengineering, 120(7), 2027-2038. https://doi.org/10.1002/bit.28426

A microfluidic lung‐on‐a‐chip based on biomimetic hydrogel membrane

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