Cancer‐upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV‐3 cells

Sunhee Lee; Wansoo Koh; Hyun‐Taek Kim; Cheol‐Hee Kim; Soojin Lee

doi:10.1002/cbf.1678

journal article Jul 21, 2010

Cancer‐upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV‐3 cells

Sunhee Lee

Wansoo Koh Hyun‐Taek Kim Cheol‐Hee Kim Soojin Lee

Cell Biochemistry and Function Vol. 28 No. 6 pp. 461-468 · Wiley

View at Publisher Save 10.1002/cbf.1678

Abstract

AbstractCancer‐upregulated gene 2 (CUG2) was originally identified as a potential oncogene commonly up‐regulated in various human cancers. Recently, CUG2 was also identified as a new member of a centromere protein complex, important in the formation of a functional kinetochore complex. Presently, we report the pro‐apoptotic effect of CUG2 when this gene was overexpressed in the SKOV‐3 human ovarian cancer cell line. Apoptotic cell death mediated by CUG2 overexpression was independently demonstrated using cell viability determination, flow cytometry analysis, chromosome fragmentation assay, and the cleavage of the death substrate poly(ADP‐ribose) polymerase. Moreover, activation of caspase‐3 and ‐8 and the cytoplasmic translocation of mitochondrial cytochrome c were evident upon CUG2 expression. Apoptotic cell death was also observed during early development of zebrafish when CUG2 was overexpressed in zebrafish embryo. We propose that high expression of CUG2 induces apoptotic cell death. Copyright © 2010 John Wiley & Sons, Ltd.

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Details

Published: Jul 21, 2010
Vol/Issue: 28(6)
Pages: 461-468
License: View

Authors

Cite This Article

Sunhee Lee, Wansoo Koh, Hyun‐Taek Kim, et al. (2010). Cancer‐upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV‐3 cells. Cell Biochemistry and Function, 28(6), 461-468. https://doi.org/10.1002/cbf.1678

Cancer‐upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV‐3 cells

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