Effect of HMGB1 silencing on cell proliferation, invasion and apoptosis of MGC‐803 gastric cancer cells

Bao Song; Wei‐guo Song; Zeng‐jun Li; Zhong‐fa Xu; Xing‐wu Wang; Chuan‐xi Wang; Jie Liu

doi:10.1002/cbf.1811

journal article Sep 27, 2011

Effect of HMGB1 silencing on cell proliferation, invasion and apoptosis of MGC‐803 gastric cancer cells

Bao Song Wei‐guo Song Zeng‐jun Li Zhong‐fa Xu Xing‐wu Wang Chuan‐xi Wang Jie Liu

Cell Biochemistry and Function Vol. 30 No. 1 pp. 11-17 · Wiley

View at Publisher Save 10.1002/cbf.1811

Abstract

High‐mobility group box 1 (HMGB1) is a multifunctional protein with intranuclear and extracellular functions. Although HMGB1 is overexpressed in approximately 85% of gastric cancers, the role of HMGB1 in gastric cancer biology remains unclear. In this study, we investigate the effect of downregulation of HMGB1 on the biological behavior of gastric cancer cells. MGC‐803 gastric cancer cells were transduced with HMGB1‐specific RNAi lentiviral vectors. Real‐time polymerase chain reaction and Western blot analysis of HMGB1 mRNA and protein, respectively, validated the silencing effects. HMGB1‐specific silencing significantly decreased cell proliferation. The impact on proliferation was observed at the cell cycle level—the number of cells in the G0/G1 phase increased, whereas that in S and G2/M phases decreased. Cell cycle changes were accompanied by decreases in cyclin D1 expression. Furthermore, HMGB1 silencing sensitized cells to apoptosis that was induced by oxaliplatin and mediated by the caspase‐3 pathway. Finally, silencing of HMGB1 expression significantly reduced cellular metastatic ability and MMP‐9 expression in MGC‐803 cells. In summary, HMGB1 not only plays an essential role in the proliferation and invasion of MGC‐803 cells but also represents a potential target for the therapeutic intervention of gastric cancer. Copyright © 2011 John Wiley & Sons, Ltd.

Topics

No keywords indexed for this article. Browse by subject →

References

29

[1]

10.1177/09680519010070041401

[2]

10.1126/science.285.5425.248

[3]

10.1038/35012626

[4]

10.1073/pnas.1014557107

[5]

10.1158/0008‐5472.can‐10‐0204

[6]

10.1016/j.bbagrm.2009.11.014

[7]

10.1186/1471‐2199‐10‐24

[8]

10.1111/j.1745‐7254.2007.00669.x

[9]

10.1016/s0002-9440(10)62296-1

[10]

10.1002/ijc.11016

[11]

10.1002/pros.20219

[12]

Kawahara N "Enhanced coexpression of thioredoxin and high mobility group protein 1 genes in human hepatocellular carcinoma and the possible association with decreased sensitivity to cisplatin" Cancer Res (1996)

[13]

10.1165/rcmb.2009‐0269oc

[14]

Akaike H "Expression of high mobility group box chromosomal protein‐1 (HMGB‐1) in gastric cancer" Anticancer Res (2007)

[15]

10.1002/(sici)1097‐0215(19970220)

[16]

10.1002/path.1031

[17]

10.1186/1479‐5876‐7‐38

[18]

10.1128/mcb.23.8.2991‐2998.2003

[19]

10.1152/ajpcell.00322.2002

[20]

10.1083/jcb.200704015

[21]

10.1038/aps.2008.8

[22]

10.1002/cbf.1735

[23]

10.1002/cbf.1754.

[24]

10.1517/13543780903292626

[25]

10.1096/fj.02‐0621fje

[26]

10.1124/mol.107.041764

[27]

10.1038/onc.2010.261

[28]

10.3748/wjg.v16.i12.1500

[29]

Takada M "Expression of receptor for advanced glycation end products (RAGE) and MMP‐9 in human pancreatic cancer cells" Hepatogastroenterology (2004)

Metrics

32

Citations

29

References

Details

Published: Sep 27, 2011
Vol/Issue: 30(1)
Pages: 11-17
License: View

Authors

B

Bao Song

Cancer Research Center, Shandong Provincial Key Laboratory of Radiation Oncology Shandong Cancer Hospital & Institute, Shandong Academy of Medical Sciences Jinan Shandong China

W

Wei‐guo Song

Department of Pharmacology Shandong Institute of Traditional Chinese Medicine Jinan Shandong China

Z

Zeng‐jun Li

Department of Oncology Shandong Cancer Hospital & Institute, Shandong Academy of Medical Sciences Jinan Shandong China

Z

Zhong‐fa Xu

Department of Oncology Shandong Cancer Hospital & Institute, Shandong Academy of Medical Sciences Jinan Shandong China

X

Xing‐wu Wang

Cancer Research Center, Shandong Provincial Key Laboratory of Radiation Oncology Shandong Cancer Hospital & Institute, Shandong Academy of Medical Sciences Jinan Shandong China

C

Chuan‐xi Wang

Department of Oncology Shandong Provincial Hospital Jinan Shandong China

J

Jie Liu

Department of Oncology Shandong Cancer Hospital & Institute, Shandong Academy of Medical Sciences Jinan Shandong China

Cite This Article

Bao Song, Wei‐guo Song, Zeng‐jun Li, et al. (2011). Effect of HMGB1 silencing on cell proliferation, invasion and apoptosis of MGC‐803 gastric cancer cells. Cell Biochemistry and Function, 30(1), 11-17. https://doi.org/10.1002/cbf.1811

Effect of HMGB1 silencing on cell proliferation, invasion and apoptosis of MGC‐803 gastric cancer cells

You May Also Like