Matcha‐silver nanoparticles reduce gamma radiation‐induced oxidative and inflammatory responses by activating SIRT1 and NLRP‐3 signaling pathways in the Wistar rat spleen

Noha Sayed Hamed; Eman F. S. Taha; Sahar Khateeb

doi:10.1002/cbf.3844

journal article Aug 31, 2023

Matcha‐silver nanoparticles reduce gamma radiation‐induced oxidative and inflammatory responses by activating SIRT1 and NLRP‐3 signaling pathways in the Wistar rat spleen

Noha Sayed Hamed

Eman F. S. Taha

Sahar Khateeb

Cell Biochemistry and Function Vol. 41 No. 8 pp. 1115-1132 · Wiley

View at Publisher Save 10.1002/cbf.3844

Abstract

AbstractThe biogenic synthesis of nanoparticles has drawn significant attention. The spleen is the largest lymphatic organ that is adversely impacted during irradiation. The current study was designated to evaluate the possible anti‐inflammatory effect of matcha‐silver nanoparticles (M‐AgNPs) to reduce inflammation associated with γ‐radiation induced‐oxidative stress and inflammation in rats' spleen. Silver nanoparticles (AgNPs) were synthesized by biogenic synthesis using a green sonochemical method from matcha (M) green tea. The obtained M‐AgNPs were extensively characterized by dynamic light scattering, transmission electron microscopy, thermogravimetric analysis, and Fourier‐transform infrared spectroscopy. Using zetasizer analysis, the surface charge, particle size, and radical scavenging DPPH assay of M‐AgNPs were also examined. Biocompatibility and cytotoxicity were analyzed by MTT assay, and the IC50 was calculated. Four groups of 24 Wistar rats each had an equal number of animals. The next step involved measuring the levels of oxidative stress markers in the rat splenic tissue. Additionally, the amounts of inflammatory protein expression were evaluated using the ELISA analysis. The results indicated the formation of spherical nanoparticles of pure Ag° coated with matcha polyphenols at the nanoscale, as well as uniform monodisperse particles suited for cellular absorption. Results revealed that M‐AgNPs improved all biochemical parameters. Furthermore, M‐AgNPs relieve inflammation by reducing the expression of NOD‐like receptor family pyrin domain‐containing 3 (NLRP3), interleukin‐1β (IL‐1β), and enhancing the levels of ileSnt information regulator 1 (SIRT1). Histopathological examinations demonstrated the ability of M‐AgNPs to overcome the damage consequent to irradiation and recover the spleen's cellular structure. These results confirmed that matcha is a potential biomaterial for synthesizing AgNPs, which can be exploited for their anti‐inflammatory activity.

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Details

Published: Aug 31, 2023
Vol/Issue: 41(8)
Pages: 1115-1132
License: View

Authors

N

Noha Sayed Hamed

Radioisotopes Department, Nuclear Research Centre Egyptian Atomic Energy Authority (EAEA) Cairo Egypt

E

Eman F. S. Taha

Health Radiation Research Department, National Centre for Radiation Research and Technology Egyptian Atomic Energy Authority (EAEA) Cairo Egypt

S

Sahar Khateeb

Department of Chemistry, Biochemistry Division, Faculty of Science Fayum University Fayum Egypt

Cite This Article

Noha Sayed Hamed, Eman F. S. Taha, Sahar Khateeb (2023). Matcha‐silver nanoparticles reduce gamma radiation‐induced oxidative and inflammatory responses by activating SIRT1 and NLRP‐3 signaling pathways in the Wistar rat spleen. Cell Biochemistry and Function, 41(8), 1115-1132. https://doi.org/10.1002/cbf.3844

Matcha‐silver nanoparticles reduce gamma radiation‐induced oxidative and inflammatory responses by activating SIRT1 and NLRP‐3 signaling pathways in the Wistar rat spleen

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