Antibody‐Directed Cancer Therapy Using Cubic‐Shaped Magnetic Nanoparticles for Combined Hyperthermia and Drug Release

S. S. Pawar; O. Selyshchev; L. Rasabathina; V. M. Khot; O. Hellwig; V. V. Kedage; D. R. T. Zahn; G. Salvan; A. D. Chougale; P. B. Patil

doi:10.1002/cmdc.202500632

journal article Jan 01, 2026

Antibody‐Directed Cancer Therapy Using Cubic‐Shaped Magnetic Nanoparticles for Combined Hyperthermia and Drug Release

S. S. Pawar

O. Selyshchev L. Rasabathina

V. M. Khot

O. Hellwig

V. V. Kedage

D. R. T. Zahn G. Salvan A. D. Chougale

P. B. Patil

ChemMedChem Vol. 21 No. 2 · Wiley

View at Publisher Save 10.1002/cmdc.202500632

Abstract

Magnetic nanoparticles (MNPs) have emerged as a promising tool for cancer therapy, providing significant potential for multimodal cancer treatments that include chemotherapy, magnetic hyperthermia, and bioactive targeting. The physicochemical properties of MNPs, including size, surface chemistry, and magnetic properties, play a crucial role in determining their therapeutic effectiveness and overall performance in multimodal cancer therapy. The present study introduces a magnetic nanoformulation (
c
MNP‐mPEG‐Dox‐Ab) nanobioengineered for an integrated therapeutic approach. The cubic‐shaped MNPs (
c
MNP) are functionalized with a biocompatible polymer, mPEG‐NH
2
, to immobilize anti‐HER2 antibody and the anticancer drug doxorubicin. The nanoformulation exhibits a controlled drug release in response to pH and temperature stimuli. Notably, under an alternating magnetic field (AMF), 64% drug release is observed at an acidic pH, which mimics the tumor microenvironment. Cytotoxicity studies on the HCC1954 breast cancer cells reveal that the nanoformulation without an anti‐HER2 antibody induces 25% cell death, which increases to 52% upon conjugation with the anti‐HER2 antibody, confirming the bioactive targeting effect. Apoptosis studies demonstrate a significant increase in the apoptotic cell population under hyperthermic conditions relative to the physiological temperature. This study underscores the potential of
c
MNP‐mPEG‐Dox‐Ab nanoformulation to enhance the precision and therapeutic efficacy of multimodal cancer therapy through bioactive targeting.

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Details

Published: Jan 01, 2026
Vol/Issue: 21(2)
License: View

Authors

S

S. S. Pawar

Department of Physics The New College Shivaji University Kolhapur Maharashtra 416012 India

O

O. Selyshchev

Semiconductor Physics Chemnitz University of Technology 09107 Chemnitz Germany

L

L. Rasabathina

Functional Magnetic Materials Chemnitz University of Technology 09107 Chemnitz Germany

V

V. M. Khot

Department of Medical Physics Centre of Interdisciplinary Research D. Y. Patil Education Society Kolhapur 416003 Maharashtra India

O

O. Hellwig

Functional Magnetic Materials Chemnitz University of Technology 09107 Chemnitz Germany; Center for Materials Architectures, and Integration of Nanomembranes (MAIN) Chemnitz University of Technology 09107 Chemnitz Germany

V

V. V. Kedage

Regrow Biosciences Pvt Ltd Lonavala Maharashtra 41040 India

D

D. R. T. Zahn

Semiconductor Physics Chemnitz University of Technology 09107 Chemnitz Germany; Center for Materials Architectures, and Integration of Nanomembranes (MAIN) Chemnitz University of Technology 09107 Chemnitz Germany

G

G. Salvan

Semiconductor Physics Chemnitz University of Technology 09107 Chemnitz Germany; Center for Materials Architectures, and Integration of Nanomembranes (MAIN) Chemnitz University of Technology 09107 Chemnitz Germany

A

A. D. Chougale

Department of Chemistry The New College Shivaji University Kolhapur Maharashtra 416012 India

P

P. B. Patil

Department of Physics The New College Shivaji University Kolhapur Maharashtra 416012 India

Funding

Deutsche Forschungsgemeinschaft Award: DFG‐TRR‐386 B03

Cite This Article

S. S. Pawar, O. Selyshchev, L. Rasabathina, et al. (2026). Antibody‐Directed Cancer Therapy Using Cubic‐Shaped Magnetic Nanoparticles for Combined Hyperthermia and Drug Release. ChemMedChem, 21(2). https://doi.org/10.1002/cmdc.202500632

Antibody‐Directed Cancer Therapy Using Cubic‐Shaped Magnetic Nanoparticles for Combined Hyperthermia and Drug Release

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