Synaptic localization of P2X7 receptors in the rat retina

Theresa Puthussery; Erica L. Fletcher

doi:10.1002/cne.20045

journal article Mar 10, 2004

Synaptic localization of P2X7 receptors in the rat retina

Theresa Puthussery Erica L. Fletcher

Journal of Comparative Neurology Vol. 472 No. 1 pp. 13-23 · Wiley

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Abstract

AbstractThe distribution of P2X7 receptor (P2X7R) subunits was studied in the rat retina using a subunit‐specific antiserum. Punctate immunofluorescence was observed in the inner and outer plexiform layers. Double labeling of P2X7 and the horizontal cell marker, calbindin, revealed extensive colocalization in the outer plexiform layer (OPL). Significant colocalization of P2X7R and kinesin, a marker of photoreceptor ribbons, was also observed, indicating that this receptor may be expressed at photoreceptor terminals. Furthermore, another band of P2X7R puncta was identified below the level of the photoreceptor terminals, adjacent to the inner nuclear layer (INL). This band of P2X7R puncta colocalized with the active‐zone protein, bassoon, suggesting that “synapse‐like” structures exist outside photoreceptor terminals. Preembedding immunoelectron microscopy demonstrated P2X7R labeling of photoreceptor terminals adjacent to ribbons. In addition, some horizontal cell dendrites and putative “desmosome‐like” junctions below cone pedicles were labeled. In the inner plexiform layer (IPL), P2X7R puncta were observed surrounding terminals immunoreactive for protein kinase C‐α, a marker of rod bipolar cells. Double labeling with bassoon in the IPL revealed extensive colocalization, indicating that P2X7R is likely to be found at conventional cell synapses. This finding was confirmed at the ultrastructural level: only processes presynaptic to rod bipolar cells were found to be labeled for the P2X7R, as well as other conventional synapses. These findings suggest that purines play a significant role in neurotransmission within the retina, and may modulate both photoreceptor and rod bipolar cell responses. J. Comp. Neurol. 472:13–23, 2004. © 2004 Wiley‐Liss, Inc.

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References

Details

Published: Mar 10, 2004
Vol/Issue: 472(1)
Pages: 13-23
License: View

Authors

Department of Anatomy and Physiology, University of Melbourne, Parkville, 3010 VIC, Australia;

Cite This Article

Theresa Puthussery, Erica L. Fletcher (2004). Synaptic localization of P2X7 receptors in the rat retina. Journal of Comparative Neurology, 472(1), 13-23. https://doi.org/10.1002/cne.20045

Synaptic localization of P2X7 receptors in the rat retina

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