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journal article Feb 09, 2013

Lack of caspase‐3 attenuates immobilization‐induced muscle atrophy and loss of tension generation along with mitigation of apoptosis and inflammation

Shimei Zhu Michio Nagashima Mohammed A.S. Khan Shingo Yasuhara Masao Kaneki J.A. Jeevendra Martyn
Muscle & Nerve Vol. 47 No. 5 pp. 711-721 · Wiley
View at Publisher Save 10.1002/mus.23642
Abstract
Introduction: Immobilization by casting induces disuse muscle atrophy (DMA). Methods: Using wild type (WT) and caspase‐3 knockout (KO) mice, we evaluated the effect of caspase‐3 on muscle mass, apoptosis, and inflammation during DMA. Results: Caspase‐3 deficiency significantly attenuated muscle mass decrease [gastrocnemius: 28 ± 1% in KO vs. 41 ± 3% in WT; soleus: 47 ± 2% in KO vs. 56 ± 2% in WT; (P < 0.05)] and gastrocnemius twitch tension decrease (23 ± 4% in KO vs. 36 ± 3% in WT, P < 0.05) at day 14 in immobilized vs. contralateral hindlimb. Lack of caspase‐3 decreased immobilization‐induced increased apoptotic myonuclei (3.2‐fold) and macrophage infiltration (2.2‐fold) in soleus muscle and attenuated increased monocyte chemoattractant protein‐1 mRNA expression (2‐fold in KO vs. 18‐fold in WT) in gastrocnemius. Conclusions: Caspase‐3 plays a key role in DMA and associated decreased tension, presumably by acting on the apoptosis and inflammation pathways. Muscle Nerve 47: 711–721, 2013
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Metrics
44
Citations
47
References
Details
Published
Feb 09, 2013
Vol/Issue
47(5)
Pages
711-721
License
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Authors
S
Shimei Zhu

Department of Anesthesia Critical Care and Pain Medicine Massachusetts General Hospital Shriners Hospitals for Children Room 206, 5l Blossom Street Boston Massachusetts 02114 USA; Harvard Medical School Boston Massachusetts 02114 USA

M
Michio Nagashima

Department of Anesthesia Critical Care and Pain Medicine Massachusetts General Hospital Shriners Hospitals for Children Room 206, 5l Blossom Street Boston Massachusetts 02114 USA; Harvard Medical School Boston Massachusetts 02114 USA

M
Mohammed A.S. Khan

Department of Anesthesia Critical Care and Pain Medicine Massachusetts General Hospital Shriners Hospitals for Children Room 206, 5l Blossom Street Boston Massachusetts 02114 USA; Harvard Medical School Boston Massachusetts 02114 USA

S
Shingo Yasuhara

Department of Anesthesia Critical Care and Pain Medicine Massachusetts General Hospital Shriners Hospitals for Children Room 206, 5l Blossom Street Boston Massachusetts 02114 USA; Harvard Medical School Boston Massachusetts 02114 USA

M
Masao Kaneki

Department of Anesthesia Critical Care and Pain Medicine Massachusetts General Hospital Shriners Hospitals for Children Room 206, 5l Blossom Street Boston Massachusetts 02114 USA; Harvard Medical School Boston Massachusetts 02114 USA

J
J.A. Jeevendra Martyn

Department of Anesthesia Critical Care and Pain Medicine Massachusetts General Hospital Shriners Hospitals for Children Room 206, 5l Blossom Street Boston Massachusetts 02114 USA; Harvard Medical School Boston Massachusetts 02114 USA

Cite This Article
Shimei Zhu, Michio Nagashima, Mohammed A.S. Khan, et al. (2013). Lack of caspase‐3 attenuates immobilization‐induced muscle atrophy and loss of tension generation along with mitigation of apoptosis and inflammation. Muscle & Nerve, 47(5), 711-721. https://doi.org/10.1002/mus.23642
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