High‐Throughput Transfection of Interfering RNA into a 3D Cell‐Culture Chip

Haiyuan Zhang; Moo‐Yeal Lee; Michael G. Hogg; Jonathan S. Dordick; Susan T. Sharfstein

doi:10.1002/smll.201102205

journal article Apr 17, 2012

High‐Throughput Transfection of Interfering RNA into a 3D Cell‐Culture Chip

Haiyuan Zhang

Moo‐Yeal Lee Michael G. Hogg Jonathan S. Dordick

Susan T. Sharfstein

Small Vol. 8 No. 13 pp. 2091-2098 · Wiley

View at Publisher Save 10.1002/smll.201102205

Abstract

AbstractA method for high‐throughput retroviral transfection of genes and interfering RNA into 3D cell‐culture microarrays is described. 3D cultures more closely mimic the in vivo cellular milieu, thus providing cellular responses to genetic manipulation more similar to the in vivo situation than 2D cultures. This technique is applied to transfect several “toxic” short‐hairpin RNAs (shRNAs) into 3D cell cultures. It is demonstrated that the toxicity is similar to that obtained by conventional (non‐high‐throughput) retroviral transfection of cells grown in similar 3D culture microarrays.

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Details

Published: Apr 17, 2012
Vol/Issue: 8(13)
Pages: 2091-2098
License: View

Authors

H

Haiyuan Zhang

Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, 130022, China; University of Science and Technology of China, Hefei, Anhui, 230026, China

M

Moo‐Yeal Lee

Biomedical Engineering Department University of North Texas Denton Texas USA

Department of Chemical and Biological Engineering and Rensselaer Nanotechnology Center, Rensselaer Polytechnic Institute, Troy, New York 12180

S

Susan T. Sharfstein

College of Nanoscale Science and Engineering SUNY Polytechnic Institute Albany New York USA

Cite This Article

Haiyuan Zhang, Moo‐Yeal Lee, Michael G. Hogg, et al. (2012). High‐Throughput Transfection of Interfering RNA into a 3D Cell‐Culture Chip. Small, 8(13), 2091-2098. https://doi.org/10.1002/smll.201102205

High‐Throughput Transfection of Interfering RNA into a 3D Cell‐Culture Chip

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