The fission yeast MAPK Spc1 senses perturbations in Cdc25 and Wee1 activities and targets Rad24 to restore this balance

Madhurima Paul; Agamani Ghosal; Sushobhana Bandyopadhyay; Prakadeeswari G.; Upasna Selvam; Neeraj Rai; Geetanjali Sundaram

doi:10.1002/yea.3289

journal article Dec 11, 2017

The fission yeast MAPK Spc1 senses perturbations in Cdc25 and Wee1 activities and targets Rad24 to restore this balance

Madhurima Paul Agamani Ghosal Sushobhana Bandyopadhyay Prakadeeswari G. Upasna Selvam Neeraj Rai

Geetanjali Sundaram

Yeast Vol. 35 No. 3 pp. 261-271 · Wiley

View at Publisher Save 10.1002/yea.3289

Abstract

AbstractMitogen‐activated protein kinases (MAPKs) play vital roles in multiple cellular processes and represent prominently pursued targets for development of therapeutic regimes. The MAPK Spc1 (p38 homologue) is known to be very important for both mitotic promotion and delay in Schizosaccharomyces pombe. However, the mechanism responsible for mitotic inhibition has remained elusive. Cdc25 (Cdc2 activator) and Wee1 (Cdc2 inhibtor) are important determinants of mitotic timing in all eukaryotes. Our results show that Spc1 can sense the perturbations in the balance of Cdc25 and Wee1 activities in S. pombe and that its function as a mitotic inhibitor is very important for controlling the same. An Spc1–Srk1–Rad24‐dependent pathway for mitotic inhibition has been reported earlier.Here we report the presence of an alternative mechanism wherein Spc1 targets the 14–3–3 protein, Rad24, independently of Srk1, leading to relocalization of Cdc25 and mitotic inhibition. Our observations suggest that this pathway can serve as a backup mechanism for Cdc2 inactivation in the absence of Wee1.

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References

37

[1]

10.1128/ec.00059-14

[2]

10.1016/s0959-437x(01)00270-2

[3]

10.1038/35075107

[4]

Chen L. "Association of Chk1 with 14–3–3 proteins is stimulated by DNA damage" Genes & Development (1999) 10.1101/gad.13.6.675

[5]

10.1091/mbc.e02-08-0499

[6]

10.1074/jbc.m109.040840

[7]

10.1128/mcb.17.6.3356

[8]

10.1128/mcb.16.6.2870

[9]

10.1093/toxsci/kfs179

[10]

10.1242/jcs.049387

[11]

10.1038/ng2033

[12]

10.4161/cc.5.16.3010

[13]

10.1242/jcs.00908

[14]

Lenassi M. "The role of p38 MAP kinase in cancer cell apoptosis" Radiology and Oncology (2006)

[15]

10.1038/sj.onc.1207188

[16]

10.1016/j.molcel.2004.11.043

[17]

10.1038/16488

[18]

10.4161/cc.4.1.1357

[19]

10.1101/gad.9.17.2117

[20]

10.1002/j.1460-2075.1991.tb05008.x

[21]

10.1016/0076-6879(91)94059-l

[22]

Paul M. "Genome wide transcription profiling of the effects of overexpression of Spc1 and its kinase dead mutant in Schizosaccharomyces pombe" Genom Data (2015) 10.1016/j.gdata.2015.10.007

[23]

10.1126/science.277.5331.1501

[24]

10.1038/nature03590

[25]

10.1038/ncb1646

[26]

NIH Image to ImageJ: 25 years of image analysis

Caroline A Schneider, Wayne S Rasband, Kevin W Eliceiri

Nature Methods 10.1038/nmeth.2089

[27]

10.1038/378739a0

[28]

10.1101/gad.10.18.2276

[29]

Shiozaki K. "Mcs4 mitotic catastrophe suppressor regulates the fission yeast cell cycle through the Wik1–Wis1–Spc1 kinase cascade" Molecular Biology of the Cell (1998) 10.1091/mbc.8.3.409

[30]

10.1091/mbc.9.6.1339

[31]

10.1074/jbc.m204593200

[32]

Sundaram G. "MAPK mediated cell cycle regulation is associated with Cdc25 turnover in S. pombe after exposure to genotoxic stress" Cell Cycle (2008) 10.4161/cc.7.3.5266

[33]

10.1002/j.1460-2075.1993.tb06017.x

[34]

10.1038/nrc2694

[35]

10.1016/j.cell.2006.11.053

[36]

Zeng Y. "DNA damage and replication checkpoints in fission yeast require nuclear exclusion of the Cdc25 phosphatase via 14–3–3 binding" Molecular and Cellular Biology (1999) 10.1128/mcb.19.11.7410

[37]

10.1038/emboj.2009.407

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Details

Published: Dec 11, 2017
Vol/Issue: 35(3)
Pages: 261-271
License: View

Authors

M

Madhurima Paul