Proteus mirabilis
                    (
                    MJA
                    2.
                    6S
                    ) from saline‐tolerant tilapia exhibits potent antagonistic activity against
                    Vibrio
                    spp., enhances immunity, controls
                    
                      NH
                      3
                    
                    levels and improves growth and survival in juvenile giant tiger shrimp,
                    Penaeus monodon

Mary Jane S. Apines‐Amar; Christopher Marlowe A. Caipang; James David M. Lopez; Ma. Novie A. Murillo; Edgar C. Amar; Lily Anne G. Piñosa; Fiona L. Pedroso

doi:10.1111/are.16033

journal article Aug 10, 2022

Proteus mirabilis ( MJA 2. 6S ) from saline‐tolerant tilapia exhibits potent antagonistic activity against Vibrio spp., enhances immunity, controls NH 3 levels and improves growth and survival in juvenile giant tiger shrimp, Penaeus monodon

Mary Jane S. Apines‐Amar

Christopher Marlowe A. Caipang

James David M. Lopez Ma. Novie A. Murillo Edgar C. Amar

Lily Anne G. Piñosa Fiona L. Pedroso

Aquaculture Research Vol. 53 No. 16 pp. 5510-5520 · Wiley

View at Publisher Save 10.1111/are.16033

Abstract

Abstract

Of several isolates from saline‐tolerant tilapia exhibiting anti‐vibrio activity, an isolate that demonstrated inhibitory activity with the biggest zone of inhibition against the shrimp pathogens,
Vibrio harveyi
and
V
.
parahaemolyticus
were chosen and investigated in this study. The isolate showed significant inhibition against the shrimp pathogens,
V
.
harveyi
and
V
.
parahaemolyticus
in a competitive assay. Upon 16S rRNA sequencing, the isolate was identified as putative
Proteus mirabilis
(MJA 2.6S).
P
.
mirabilis
(MJA 2.6S) exhibited extracellular protease, cellulase and amylase activities. Pathogenicity tests showed that it was not pathogenic to the shrimp even at the highest concentration of 10
8
CFU ml
−1
. Further,
P
.
mirabilis
(MJA 2.6S) improved growth and survival, stimulated higher phenoloxidase and bactericidal activities in the shrimp host and protected it from
V
.
harveyi
experimental infection. In addition, the isolate
P
.
mirabilis
(MJA 2.6S) significantly reduced the ammonia level of the pond water samples in vitro. Taken together, the results clearly showed the strong probiotic potential of
P
.
mirabilis
(MJA 2.6S) in shrimp and hence further studies are needed to determine its wider application as a probiotic in
P
.
monodon
culture.

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Details

Published: Aug 10, 2022
Vol/Issue: 53(16)
Pages: 5510-5520
License: View

Authors

M

Mary Jane S. Apines‐Amar