Cigarette smoking differentially affects immunoglobulin class levels in serum and saliva: An investigation and review

Nesrin Tarbiah; Ian Todd; Patrick J. Tighe; Lucy C. Fairclough

doi:10.1111/bcpt.13278

journal article Jul 21, 2019

Cigarette smoking differentially affects immunoglobulin class levels in serum and saliva: An investigation and review

Nesrin Tarbiah Ian Todd Patrick J. Tighe

Lucy C. Fairclough

Basic & Clinical Pharmacology & Toxicology Vol. 125 No. 5 pp. 474-483 · Wiley

View at Publisher Save 10.1111/bcpt.13278

Abstract

AbstractThe aim of the present study was to compare concentrations of IgG, IgA, IgM and IgD in both serum and saliva samples from smoking and non‐smoking individuals using a protein microarray assay. The findings were also compared to previous studies. Serum and saliva were collected from 48 smoking male individuals and 48 age‐matched never‐smoker male individuals. The protein microarray assays for detection of human IgG, IgM, IgA and IgD were established and optimized using Ig class‐specific affinity‐purified goat anti‐human Ig‐Fc capture antibodies and horseradish peroxidase (HRP)‐conjugated goat anti‐human Ig‐Fc detection antibodies. The Ig class specificity of the microarray assays was verified, and the optimal dilutions of serum and saliva samples were determined for quantification of Ig levels against standard curves. We found that smoking is associated with reduced IgG concentrations and enhanced IgA concentrations in both serum and saliva. By contrast, smoking differentially affected IgM concentrations—causing increased concentrations in serum, but decreased concentrations in saliva. Smoking was associated with decreased IgD concentrations in serum and did not have a significant effect on the very low IgD concentrations in saliva. Thus, cigarette smoking differentially affects the levels of Ig classes systemically and in the oral mucosa. Although there is variation between the results of different published studies, there is a consensus that smokers have significantly reduced levels of IgG in both serum and saliva. A functional antibody deficiency associated with smoking may compromise the body's response to infection and result in a predisposition to the development of autoimmunity.

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Details

Published: Jul 21, 2019
Vol/Issue: 125(5)
Pages: 474-483
License: View

Authors

N

Nesrin Tarbiah

School of Life Sciences University of Nottingham Nottingham UK

I

Ian Todd

School of Life Sciences University of Nottingham Nottingham UK

P

Patrick J. Tighe

School of Life Sciences University of Nottingham Nottingham UK

L

Lucy C. Fairclough

School of Life Sciences University of Nottingham Nottingham UK

Funding

The Jones 1986 Charitable Trust

Cite This Article

Nesrin Tarbiah, Ian Todd, Patrick J. Tighe, et al. (2019). Cigarette smoking differentially affects immunoglobulin class levels in serum and saliva: An investigation and review. Basic & Clinical Pharmacology & Toxicology, 125(5), 474-483. https://doi.org/10.1111/bcpt.13278

Cigarette smoking differentially affects immunoglobulin class levels in serum and saliva: An investigation and review

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