Transplantation of tauroursodeoxycholic acid–inducing M2‐phenotype macrophages promotes an anti‐neuroinflammatory effect and functional recovery after spinal cord injury in rats

Gong Ho Han; Seong Jun Kim; Wan‐Kyu Ko; Daye Lee; In‐Bo Han; Seung Hun Sheen; Je Beom Hong; Seil Sohn

doi:10.1111/cpr.13050

journal article Open Access May 07, 2021

Transplantation of tauroursodeoxycholic acid–inducing M2‐phenotype macrophages promotes an anti‐neuroinflammatory effect and functional recovery after spinal cord injury in rats

Gong Ho Han Seong Jun Kim Wan‐Kyu Ko Daye Lee In‐Bo Han Seung Hun Sheen Je Beom Hong Seil Sohn

Cell Proliferation Vol. 54 No. 6 · Wiley

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Abstract

AbstractObjectivesIn this study, we study the transplantation of tauroursodeoxycholic acid (TUDCA)‐induced M2‐phenotype (M2) macrophages and their ability to promote anti‐neuroinflammatory effects and functional recovery in a spinal cord injury (SCI) model.MethodsTo this end, compared to the granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), we evaluated whether TUDCA effectively differentiates bone marrow–derived macrophages (BMDMs) into M2 macrophages.ResultsThe M2 expression markers in the TUDCA‐treated BMDM group were increased more than those in the GM‐CSF‐treated BMDM group. After the SCI and transplantation steps, pro‐inflammatory cytokine levels and the mitogen‐activated protein kinase (MAPK) pathway were significantly decreased in the TUDCA‐induced M2 group more than they were in the GM‐CSF‐induced M1 group and in the TUDCA group. Moreover, the TUDCA‐induced M2 group showed significantly enhanced tissue volumes and improved motor functions compared to the GM‐CSF‐induced M1 group and the TUDCA group. In addition, biotinylated dextran amine (BDA)–labelled corticospinal tract (CST) axons and neuronal nuclei marker (NeuN) levels were increased in the TUDCA‐induced M2 group more than those in the GM‐CSF‐induced M1 group and the TUDCA group.ConclusionsThis study demonstrates that the transplantation of TUDCA‐induced M2 macrophages promotes an anti‐neuroinflammatory effect and motor function recovery in SCI. Therefore, we suggest that the transplantation of TUDCA‐induced M2 macrophages represents a possible alternative cell therapy for SCI.

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Details

Published: May 07, 2021
Vol/Issue: 54(6)
License: View

Authors

G

Gong Ho Han

Department of Neurosurgery CHA Bundang Medical Center CHA University Seongnam‐si Korea; Department of Biomedical Science CHA University Seongnam‐si Korea

S

Seong Jun Kim

Department of Neurosurgery CHA Bundang Medical Center CHA University Seongnam‐si Korea; Department of Biomedical Science CHA University Seongnam‐si Korea

W

Wan‐Kyu Ko

Department of Neurosurgery CHA Bundang Medical Center CHA University Seongnam‐si Korea; Department of Biomedical Science CHA University Seongnam‐si Korea

D

Daye Lee

Department of Neurosurgery CHA Bundang Medical Center CHA University Seongnam‐si Korea; Department of Biomedical Science CHA University Seongnam‐si Korea

I

In‐Bo Han

Department of Neurosurgery CHA Bundang Medical Center CHA University Seongnam‐si Korea

S

Seung Hun Sheen

Department of Neurosurgery CHA Bundang Medical Center CHA University Seongnam‐si Korea

J

Je Beom Hong

Department of Neurosurgery Kangbuk Samsung Hospital Sungkyunkwan University School of Medicine Seoul Korea

S

Seil Sohn

Department of Neurosurgery CHA Bundang Medical Center CHA University Seongnam‐si Korea

Cite This Article

Gong Ho Han, Seong Jun Kim, Wan‐Kyu Ko, et al. (2021). Transplantation of tauroursodeoxycholic acid–inducing M2‐phenotype macrophages promotes an anti‐neuroinflammatory effect and functional recovery after spinal cord injury in rats. Cell Proliferation, 54(6). https://doi.org/10.1111/cpr.13050

Transplantation of tauroursodeoxycholic acid–inducing M2‐phenotype macrophages promotes an anti‐neuroinflammatory effect and functional recovery after spinal cord injury in rats

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