Regulatory role of human fibrocartilage stem cells in condyle osteochondroma

Qing Yin; Ruiye Bi; Haohan Li; Qianli Li; Peiran Li; Ruiyu Wang; Songsong Zhu

doi:10.1111/cpr.13342

journal article Open Access Sep 26, 2022

Regulatory role of human fibrocartilage stem cells in condyle osteochondroma

Qing Yin

Ruiye Bi Haohan Li Qianli Li Peiran Li

Ruiyu Wang Songsong Zhu

Cell Proliferation Vol. 56 No. 1 · Wiley

View at Publisher Save 10.1111/cpr.13342

Abstract

AbstractObjectiveOsteochondroma is a common benign skeletal disorder for which different molecular and histological features of long bones have been reported. We investigated cell‐of‐origin and molecular mechanisms of a rare condylar osteochondroma (CO).MethodsHuman fibrocartilage stem cells (hFCSCs) isolated from CO and normal condyle tissue were used for RNA sequencing, real‐time PCR, Western Blotting, immunohistology, flowcytometry, as well as for chondrogenic differentiation, proliferation, and apoptosis detection assays.ResultsHFCSCs were fewer in number with weaker proliferative capacity and higher apoptosis ratio in the CO group. During the chondrogenic inducing process, hFCSCs from CO were prone to form more mature and hypertrophic cartilage. The result of RNA sequencing of hFCSCs from CO and normal condyle revealed a correlation between the PI3K/AKT signalling pathway and CO. Activated PI3K/AKT signalling might lead to functional changes in hFCSCs by enhancing cell apoptosis in the developmental process of CO. Increased expression of BCL2‐like protein 11 (BIM) in CO tissue also supports this conclusion. Furthermore, the activation of the PI3K/AKT pathway in TMJ of mice induced histological disorder and increased apoptosis in condylar cartilage.ConclusionWe conclude that the activation of PI3K/AKT signalling in hFCSCs of CO suggests a new hypothesis for the cell‐of‐origin of human CO and another possible target to treat it.

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References

33

[1]

Reijnders C "Bone: Osteochondroma" Atlas Genet Cytogenet Oncol Haematol (2009)

[2]

10.1016/j.humpath.2007.01.017

[3]

10.1148/radiographics.20.5.g00se171407

[4]

10.1016/j.tripleo.2006.11.035

[5]

10.1053/joms.2003.50022

[6]

10.1016/0278-2391(95)90293-7

[7]

10.1259/dmfr/19168643

[8]

Muller E "Uber hereditare multiple cartilaginare exostosen und ecchondrosen" Beitr Pathol Anat (1914)

[9]

10.3109/17453676708989639

[10]

Rang M "The growth plate and its disorders" Br J Surg (1969)

[11]

10.1002/jor.1100130516

[12]

10.1007/bf02554930

[13]

10.1038/ncomms13073

[14]

10.1177/0022034517735094

[15]

Identification of human temporomandibular joint fibrocartilage stem cells with distinct chondrogenic capacity

R. Bi, Q. Yin, J. Mei et al.

Osteoarthritis and Cartilage 10.1016/j.joca.2020.02.835

[16]

10.1016/j.bone.2005.12.003

[17]

10.1002/path.2127

[18]

10.1302/0301-620x.86b7.14815

[19]

10.2106/jbjs.i.00391

[20]

Qin Z "Detection of EXT gene mutations in patients with sporadic osteochondroma of condyle" China J Oral Maxillofac Surg (2015)

[21]

10.1002/path.2886

[22]

10.1002/jcp.20620

[23]

10.1016/j.cellsig.2007.02.007

[24]

10.1042/bj20041636

[25]

10.1111/j.1365-2443.2008.01209.x

[26]

10.1038/sj.onc.1210274

[27]

10.1083/jcb.200401138

[28]

10.1016/j.cell.2017.07.029

[29]

10.1152/physiolgenomics.00173.2013

[30]

10.1007/s00204-014-1448-7

[31]

10.1016/s0736-0266(01)00041-9

[32]

10.1038/labinvest.3780202

[33]

10.1016/j.bone.2018.03.010

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Published: Sep 26, 2022
Vol/Issue: 56(1)
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Qing Yin