Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures

Roy U. Schenk; A. C. Hildebrandt

doi:10.1139/b72-026

journal article Jan 01, 1972

Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures

Roy U. Schenk A. C. Hildebrandt

Canadian Journal of Botany Vol. 50 No. 1 pp. 199-204 · Canadian Science Publishing

View at Publisher Save 10.1139/b72-026

Abstract

The composition of a medium is described that proved useful to culture callus of a variety of monocotyledonous and dicotyledonous plants. Growth on the medium was often better than on some other excellent media. In addition to supporting rapid cell growth, a soft, friable type of colony growth was often obtained. This type of loose, friable cell growth facilitated work with single cells and the enzymatic removal of cell walls in related studies. A high level of auxin-type growth-regulating substances (AGRS) generally favored cell cultures of monocotyledonous plants, while low levels of cytokinin were essential for most dicotyledonous cell cultures. Some cultures of dicotyledonous plant cells adapted to a cytokinin-free medium containing a high level of 2,4-dichlorophenoxyacetic acid (2,4-D) or p-chlorophenoxyacetic acid (pCPA). The preferred AGRS were 2,4-D and pCPA. Citrate, succinate, and 2(N-morpholino) ethane sulfonic acid (MES) were effective medium buffers, but phosphate alone seemed adequate to buffer the medium at pH 5.8–5.9.

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Published: Jan 01, 1972
Vol/Issue: 50(1)
Pages: 199-204
License: View

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Cite This Article

Roy U. Schenk, A. C. Hildebrandt (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany, 50(1), 199-204. https://doi.org/10.1139/b72-026

Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures

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