A method for the extraction of chlorophyll from leaf tissue without maceration

J. D. Hiscox; G. F. Israelstam

doi:10.1139/b79-163

journal article Jun 15, 1979

A method for the extraction of chlorophyll from leaf tissue without maceration

J. D. Hiscox G. F. Israelstam

Canadian Journal of Botany Vol. 57 No. 12 pp. 1332-1334 · Canadian Science Publishing

View at Publisher Save 10.1139/b79-163

Abstract

A simple, rapid method requiring few manipulations for the extraction of chlorophylls from fragmented leaf tissue of angiosperms and gymnosperms is compared with the widely used acetone method. Unlike the acetone method where grinding and subsequent centrifugation are essential, this method makes use of incubation at 65 °C of leaf tissue immersed in dimethyl sulphoxide. The new method was found to be as efficient as acetone for chlorophyll extraction and superior in terms of chlorophyll stability.

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Published: Jun 15, 1979
Vol/Issue: 57(12)
Pages: 1332-1334
License: View

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Cite This Article

J. D. Hiscox, G. F. Israelstam (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, 57(12), 1332-1334. https://doi.org/10.1139/b79-163

A method for the extraction of chlorophyll from leaf tissue without maceration

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