Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities

Nimaichand Salam; Thi-Nhan Khieu; Min-Jiao Liu; Thu-Trang Vu; Son Chu-Ky; Ngoc-Tung Quach; Quyet-Tien Phi; Manik Prabhu Narsing Rao; Angélique Fontana; Samira Sarter; Wen-Jun Li

doi:10.1155/2017/1308563

journal article Open Access Jan 01, 2017

Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities

Nimaichand Salam Thi-Nhan Khieu Min-Jiao Liu Thu-Trang Vu Son Chu-Ky Ngoc-Tung Quach Quyet-Tien Phi

Manik Prabhu Narsing Rao

Angélique Fontana

Samira Sarter

Wen-Jun Li

BioMed Research International Vol. 2017 pp. 1-11 · Wiley

View at Publisher Save 10.1155/2017/1308563

Abstract

Dracaena cochinchinensis Lour. is an ethnomedicinally important plant used in traditional Chinese medicine known as dragon’s blood. Excessive utilization of the plant for extraction of dragon’s blood had resulted in the destruction of the important niche. During a study to provide a sustainable way of utilizing the resources, the endophytic Actinobacteria associated with the plant were explored for potential utilization of their medicinal properties. Three hundred and four endophytic Actinobacteria belonging to the genera Streptomyces, Nocardiopsis, Brevibacterium, Microbacterium, Tsukamurella, Arthrobacter, Brachybacterium, Nocardia, Rhodococcus, Kocuria, Nocardioides, and Pseudonocardia were isolated from different tissues of D. cochinchinensis Lour. Of these, 17 strains having antimicrobial and anthracyclines-producing activities were further selected for screening of antifungal and cytotoxic activities against two human cancer cell lines, MCF-7 and Hep G2. Ten of these selected endophytic Actinobacteria showed antifungal activities against at least one of the fungal pathogens, of which three strains exhibited cytotoxic activities with IC50-values ranging between 3 and 33 μg·mL−1. Frequencies for the presence of biosynthetic genes, polyketide synthase- (PKS-) I, PKS-II, and nonribosomal peptide synthetase (NRPS) among these 17 selected bioactive Actinobacteria were 29.4%, 70.6%, and 23.5%, respectively. The results indicated that the medicinal plant D. cochinchinensis Lour. is a good niche of biologically important metabolites-producing Actinobacteria.

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References

49

[1]

10.1038/ja.2012.27

[2]

10.1023/b:wibi.0000023824.20673.2f

[3]

10.1155/2014/698178

[4]

10.1111/j.1472-765x.2008.02470.x

[5]

10.1099/ijs.0.007534-0

[6]

10.1007/s10482-011-9661-3

[7]

10.1128/aem.01034-09

[8]

10.1007/s00253-010-2923-6

[9]

10.1111/j.1758-2229.2012.00357.x

[10]

10.3389/fmicb.2015.00574

[11]

10.1016/j.plantsci.2010.09.007

[12]

10.1016/j.jep.2007.10.018

[13]

Zhongguo Zhongyao Zazhi (2008)

[14]

10.1099/ijs.0.64749-0

[15]

Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species

Ok-Sun Kim, Yong-Joon Cho, Kihyun Lee et al.

International Journal of Systematic and Evolutiona... 10.1099/ijs.0.038075-0

[16]

The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools

J. Thompson

Nucleic Acids Research 10.1093/nar/25.24.4876

[17]

Molecular Biology and Evolution (1987)

[18]

(1983)

[19]

MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0

Koichiro Tamura, Glen Stecher, Daniel Peterson et al.

Molecular Biology and Evolution 10.1093/molbev/mst197

[20]

Confidence Limits on Phylogenies: An Approach Using the Bootstrap

Joseph Felsenstein

Evolution 10.2307/2408678

[21]

10.1016/0305-4179(94)90035-3

[22]

(1996)

[23]

10.1017/s0950268807009326

[24]

10.1007/s11274-008-9933-x

[25]

10.1093/jnci/83.11.757

[26]

10.2174/1568026023394317

[27]

10.1080/10286020.2014.882912

[28]

10.1002/bip.21483

[29]

10.1200/jco.2001.19.1.191

[30]

10.1021/ja034781q

[31]

10.1023/a:1023901107182

[32]

10.1007/s00248-008-9450-3

[33]

10.3389/fmicb.2015.00273

[34]

10.1007/s00284-010-9685-3

[35]

10.1002/ptr.3361

[36]

10.1111/j.1472-765x.2004.01606.x

[37]

10.1099/ijs.0.64385-0

[38]

10.1007/s00248-006-9129-6

[39]

10.1099/ijs.0.65671-0

[40]

10.1094/mpmi-19-0827

[41]

10.1016/j.soilbio.2009.11.024

[42]

Bangladesh Pharmaceutical Journal (2010)

[43]

European Journal of Biological Sciences (2010)

[44]

10.1590/s1516-8913201400173

[45]

10.1038/ja.2007.84

[46]

10.1128/aem.02852-09

[47]

International Journal of Pharmacy and Pharmaceutical Sciences (2014)

[48]

10.1038/ja.2006.105

[49]

10.1016/s1074-5521(00)00011-9

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67

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49

References

Details

Published: Jan 01, 2017
Vol/Issue: 2017
Pages: 1-11
License: View

Authors

N

Nimaichand Salam

State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China

T

Thi-Nhan Khieu

State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China; Department of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, Vietnam

M

Min-Jiao Liu

Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China

T

Thu-Trang Vu

Department of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, Vietnam

S

Son Chu-Ky

Department of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, Vietnam

N

Ngoc-Tung Quach

Laboratory of Fermentation Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

Q

Quyet-Tien Phi

Laboratory of Fermentation Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

M

Manik Prabhu Narsing Rao

State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China

A

Angélique Fontana

CIRAD, UMR QUALISUD, 34398 Montpellier, France

S

Samira Sarter

CIRAD, UMR QUALISUD, 34398 Montpellier, France

W

Wen-Jun Li

State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China; Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China

Funding

China Postdoctoral Science Foundation Award: 2016M602566

Sun Yat-sen University Award: 2016M602566

Vietnam Ministry of Education and Training Award: 2016M602566

Guangdong Province Higher Vocational Colleges & Schools Award: 2016M602566

Cite This Article

Nimaichand Salam, Thi-Nhan Khieu, Min-Jiao Liu, et al. (2017). Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities. BioMed Research International, 2017, 1-11. https://doi.org/10.1155/2017/1308563

Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities

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