A component of the transcriptional mediator complex inhibits RAS-dependent vulval fate specification inC. elegans

Nadeem Moghal; Paul W. Sternberg

doi:10.1242/dev.00189

journal article Jan 01, 2003

A component of the transcriptional mediator complex inhibits RAS-dependent vulval fate specification inC. elegans

Nadeem Moghal Paul W. Sternberg

Development Vol. 130 No. 1 pp. 57-69 · The Company of Biologists

View at Publisher Save 10.1242/dev.00189

Abstract

Negative regulation of receptor tyrosine kinase (RTK)/RAS signaling pathways is important for normal development and the prevention of disease in humans. We have used a genetic screen in C. elegans to identify genes that antagonize the activity of activated LET-23, a member of the EGFR family of RTKs. We identified two loss-of-function mutations in dpy-22,previously cloned as sop-1, that promote the ability of activated LET-23 to induce ectopic vulval fates. DPY-22 is a glutamine-rich protein that is most similar to human TRAP230, a component of a transcriptional mediator complex. DPY-22 has previously been shown to regulate WNT responses through inhibition of the β-catenin-like protein BAR-1. We provide evidence that DPY-22 also inhibits RAS-dependent vulval fate specification independently of BAR-1, and probably regulates the activities of multiple transcription factors during development. Furthermore, we demonstrate that although inhibition of BAR-1-dependent gene expression has been shown to require the C-terminal glutamine-rich region, this region is dispensable for inhibition of RAS-dependent cell differentiation. Thus, the glutamine-rich region contributes to specificity of this class of mediator protein.

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Details

Published: Jan 01, 2003
Vol/Issue: 130(1)
Pages: 57-69

Authors

N

Nadeem Moghal

Howard Hughes Medical Institute, and Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA

P

Paul W. Sternberg

Howard Hughes Medical Institute, and Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA

Cite This Article

Nadeem Moghal, Paul W. Sternberg (2003). A component of the transcriptional mediator complex inhibits RAS-dependent vulval fate specification inC. elegans. Development, 130(1), 57-69. https://doi.org/10.1242/dev.00189

A component of the transcriptional mediator complex inhibits RAS-dependent vulval fate specification inC. elegans

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