journal article
Feb 15, 2007
Distinct functions of α-Spectrin and β-Spectrin during axonal pathfinding
Abstract
Cell-shape changes during development require a precise coupling of the cytoskeleton with proteins situated in the plasma membrane. Important elements controlling the shape of cells are the Spectrin proteins that are expressed as a subcortical cytoskeletal meshwork linking specific membrane receptors with F-actin fibers. Here, we demonstrate that Drosophila karussellmutations affect β-spectrin and lead to distinct axonal patterning defects in the embryonic CNS. karussell mutants display a slit-sensitive axonal phenotype characterized by axonal looping in stage-13 embryos. Further analyses of individual, labeled neuroblast lineages revealed abnormally structured growth cones in these animals. Cell-type-specific rescue experiments demonstrate that β-Spectrin is required autonomously and non-autonomously in cortical neurons to allow normal axonal patterning. Within the cell, β-Spectrin is associated withα-Spectrin. We show that expression of the two genes is tightly regulated by post-translational mechanisms. Loss of β-Spectrin significantly reduces levels of neuronal α-Spectrin expression, whereas gain of β-Spectrin leads to an increase in α-Spectrin protein expression. Because the loss of α-spectrin does not result in an embryonic nervous system phenotype, β-Spectrin appears to act at least partially independent of α-Spectrin to control axonal patterning.
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Details
- Published
- Feb 15, 2007
- Vol/Issue
- 134(4)
- Pages
- 713-722
Authors
Cite This Article
Jörn Hülsmeier, Jan Pielage, Christof Rickert, et al. (2007). Distinct functions of α-Spectrin and β-Spectrin during axonal pathfinding. Development, 134(4), 713-722. https://doi.org/10.1242/dev.02758
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