VEGF mediates angioblast migration during development of the dorsal aorta in Xenopus

Ondine Cleaver; Paul A. Krieg

doi:10.1242/dev.125.19.3905

journal article Oct 01, 1998

VEGF mediates angioblast migration during development of the dorsal aorta in Xenopus

Ondine Cleaver

Paul A. Krieg

Development Vol. 125 No. 19 pp. 3905-3914 · The Company of Biologists

View at Publisher Save 10.1242/dev.125.19.3905

Abstract

ABSTRACT
Angioblasts are precursor cells of the vascular endothelium which organize into the primitive blood vessels during embryogenesis. The molecular mechanisms underlying patterning of the embryonic vasculature remain unclear. Mutational analyses of the receptor tyrosine kinase flk-1 and its ligand vascular endothelial growth factor, VEGF, indicate that these molecules are critical for vascular development. Targeted ablation of the flk-1 gene results in complete failure of blood and vascular development (F. Shalaby et al. (1995)Nature 376, 62-66), while targeted ablation of the VEGF gene results in gross abnormalities in vascular patterning (P. Carmeliet et al. (1996)Nature 380, 435-439; N. Ferrara et al. (1996)Nature 380, 439-442). Here we report a role for VEGF in patterning the dorsal aorta of the Xenopus embryo. We show that the diffusible form of VEGF is expressed by the hypochord, which lies at the embryonic midline immediately dorsal to the location of the future dorsal aorta. We find that, initially, no flk-1- expressing angioblasts are present at this location, but that during subsequent development, angioblasts migrate from the lateral plate mesoderm to the midline where they form a single dorsal aorta. We have demonstrated that VEGF can act as a chemoattractant for angioblasts by ectopic expression of VEGF in the embryo. These results strongly suggest that localized sources of VEGF play a role in patterning the embryonic vasculature.

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Details

Published: Oct 01, 1998
Vol/Issue: 125(19)
Pages: 3905-3914
License: View

Authors

O

Ondine Cleaver

University of Texas at Austin Institute for Cellular and Molecular Biology and Department of Zoology , , Austin, TX 78712, USA

P

Paul A. Krieg

University of Texas at Austin Institute for Cellular and Molecular Biology and Department of Zoology , , Austin, TX 78712, USA

Cite This Article

Ondine Cleaver, Paul A. Krieg (1998). VEGF mediates angioblast migration during development of the dorsal aorta in Xenopus. Development, 125(19), 3905-3914. https://doi.org/10.1242/dev.125.19.3905

VEGF mediates angioblast migration during development of the dorsal aorta in Xenopus

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