The spatial relationship between stem cells and their progeny in the basal layer of human epidermis: a new view based on whole-mount labelling and lineage analysis

Uffe B. Jensen; Sally Lowell; Fiona M. Watt

doi:10.1242/dev.126.11.2409

journal article Jun 01, 1999

The spatial relationship between stem cells and their progeny in the basal layer of human epidermis: a new view based on whole-mount labelling and lineage analysis

Uffe B. Jensen Sally Lowell

Fiona M. Watt

Development Vol. 126 No. 11 pp. 2409-2418 · The Company of Biologists

View at Publisher Save 10.1242/dev.126.11.2409

Abstract

ABSTRACT
In order to examine the spatial organisation of stem cells and their progeny in human epidermis, we developed a method for whole-mount epidermal immunofluorescence labelling using high surface β1 integrin expression as a stem cell marker. We confirmed that there are clusters of high β1 integrin-expressing cells at the tips of the dermal papillae in epidermis from several body sites, whereas α6 integrin expression is more uniform. The majority of actively cycling cells detected by Ki67 or bromodeoxyuridine labelling were found in the β1 integrindull, transit amplifying population and integrin-negative, keratin 10-positive cells left the basal layer exclusively from this compartment. When we examined p53-positive clones in sun-exposed epidermis, we found two types of clone that differed in size and position in a way that was consistent with the founder cell being a stem or transit amplifying cell. The patterning of the basal layer implies that transit amplifying cells migrate over the basement membrane away from the stem cell clusters. In support of this, isolated β1 integrin-dull keratinocytes were more motile on type IV collagen than β1 integrin-bright keratinocytes and EGFP-labelled stem cell clones in confluent cultured sheets were compact, whereas transit amplifying clones were dispersed. The combination of whole-mount labelling and lineage marking thus reveals features of epidermal organisation that were previously unrecognised.

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Details

Published: Jun 01, 1999
Vol/Issue: 126(11)
Pages: 2409-2418
License: View

Authors

U

Uffe B. Jensen

Keratinocyte Laboratory, Imperial Cancer Research Fund, 44 Lincoln’s Inn Fields, London WC2A 3PX, UK

S

Sally Lowell

Keratinocyte Laboratory, Imperial Cancer Research Fund, 44 Lincoln’s Inn Fields, London WC2A 3PX, UK

F

Fiona M. Watt

Keratinocyte Laboratory, Imperial Cancer Research Fund, 44 Lincoln’s Inn Fields, London WC2A 3PX, UK

Cite This Article

Uffe B. Jensen, Sally Lowell, Fiona M. Watt (1999). The spatial relationship between stem cells and their progeny in the basal layer of human epidermis: a new view based on whole-mount labelling and lineage analysis. Development, 126(11), 2409-2418. https://doi.org/10.1242/dev.126.11.2409

The spatial relationship between stem cells and their progeny in the basal layer of human epidermis: a new view based on whole-mount labelling and lineage analysis

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