Distinct contributions of ECM proteins to basement membrane mechanical properties in Drosophila

Uwe Töpfer; Karla Yanín Guerra Santillán; Elisabeth Fischer-Friedrich; Christian Dahmann

doi:10.1242/dev.200456

journal article Open Access May 15, 2022

Distinct contributions of ECM proteins to basement membrane mechanical properties in Drosophila

Uwe Töpfer Karla Yanín Guerra Santillán Elisabeth Fischer-Friedrich

Christian Dahmann

Development Vol. 149 No. 10 · The Company of Biologists

View at Publisher Save 10.1242/dev.200456

Abstract

ABSTRACT
The basement membrane is a specialized extracellular matrix (ECM) that is crucial for the development of epithelial tissues and organs. In Drosophila, the mechanical properties of the basement membrane play an important role in the proper elongation of the developing egg chamber; however, the molecular mechanisms contributing to basement membrane mechanical properties are not fully understood. Here, we systematically analyze the contributions of individual ECM components towards the molecular composition and mechanical properties of the basement membrane underlying the follicle epithelium of Drosophila egg chambers. We find that the Laminin and Collagen IV networks largely persist in the absence of the other components. Moreover, we show that Perlecan and Collagen IV, but not Laminin or Nidogen, contribute greatly towards egg chamber elongation. Similarly, Perlecan and Collagen, but not Laminin or Nidogen, contribute towards the resistance of egg chambers against osmotic stress. Finally, using atomic force microscopy we show that basement membrane stiffness mainly depends on Collagen IV. Our analysis reveals how single ECM components contribute to the mechanical properties of the basement membrane controlling tissue and organ shape.

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Details

Published: May 15, 2022
Vol/Issue: 149(10)
License: View

Authors

U

Uwe Töpfer

Institute of Genetics 1 , , 01062 Dresden , Germany; Technische Universität Dresden 1 , , 01062 Dresden , Germany

K

Karla Yanín Guerra Santillán

Institute of Genetics 1 , , 01062 Dresden , Germany; Technische Universität Dresden 1 , , 01062 Dresden , Germany; Biotechnology Center 2 , , 01307 Dresden , Germany; Technische Universität Dresden 2 , , 01307 Dresden , Germany; Cluster of Excellence Physics of Life, Technische Universität Dresden 3 , 01062 Dresden , Germany

E

Elisabeth Fischer-Friedrich

Biotechnology Center 2 , , 01307 Dresden , Germany; Technische Universität Dresden 2 , , 01307 Dresden , Germany; Cluster of Excellence Physics of Life, Technische Universität Dresden 3 , 01062 Dresden , Germany

C

Christian Dahmann

Institute of Genetics 1 , , 01062 Dresden , Germany; Technische Universität Dresden 1 , , 01062 Dresden , Germany; Cluster of Excellence Physics of Life, Technische Universität Dresden 3 , 01062 Dresden , Germany

Funding

Deutsche Forschungsgemeinschaft Award: DA586-16/2

Technische Universität Dresden

Cite This Article

Uwe Töpfer, Karla Yanín Guerra Santillán, Elisabeth Fischer-Friedrich, et al. (2022). Distinct contributions of ECM proteins to basement membrane mechanical properties in Drosophila. Development, 149(10). https://doi.org/10.1242/dev.200456

Distinct contributions of ECM proteins to basement membrane mechanical properties in Drosophila

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