Ets-1 transcription factor regulates glial cell regeneration and function in planarians

Bidushi Chandra; Matthew G. Voas; Erin L. Davies; Rachel H. Roberts-Galbraith

doi:10.1242/dev.201666

journal article Open Access Sep 14, 2023

Ets-1 transcription factor regulates glial cell regeneration and function in planarians

Bidushi Chandra

Matthew G. Voas

Erin L. Davies

Rachel H. Roberts-Galbraith

Development Vol. 150 No. 18 · The Company of Biologists

View at Publisher Save 10.1242/dev.201666

Abstract

ABSTRACT
Glia play multifaceted roles in nervous systems in response to injury. Depending on the species, extent of injury and glial cell type in question, glia can help or hinder the regeneration of neurons. Studying glia in the context of successful regeneration could reveal features of pro-regenerative glia that could be exploited for new human therapies. Planarian flatworms completely regenerate their nervous systems after injury – including glia – and thus provide a strong model system for exploring glia in the context of regeneration. Here, we report that planarian glia regenerate after neurons, and that neurons are required for correct glial numbers and localization during regeneration. We also identify the planarian transcription factor-encoding gene ets-1 as a key regulator of glial cell maintenance and regeneration. Using ets-1 (RNAi) to perturb glia, we show that glial loss is associated with altered neuronal gene expression, impeded animal movement and impaired nervous system architecture – particularly within the neuropil. Importantly, our work reveals the inter-relationships of glia and neurons in the context of robust neural regeneration.

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Details

Published: Sep 14, 2023
Vol/Issue: 150(18)
License: View

Authors

B

Bidushi Chandra

University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602, USA

M

Matthew G. Voas

Center for Cancer Research, National Cancer Institute, National Institutes of Health 2 , Frederick, MD 21702, USA

E

Erin L. Davies

Center for Cancer Research, National Cancer Institute, National Institutes of Health 2 , Frederick, MD 21702, USA

R

Rachel H. Roberts-Galbraith

University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602, USA

Funding

National Cancer Institute

National Institutes of Health Award: ZIABC0120009

Alfred P. Sloan Foundation

McKnight Foundation

University of Georgia

Cite This Article

Bidushi Chandra, Matthew G. Voas, Erin L. Davies, et al. (2023). Ets-1 transcription factor regulates glial cell regeneration and function in planarians. Development, 150(18). https://doi.org/10.1242/dev.201666

Ets-1 transcription factor regulates glial cell regeneration and function in planarians

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