Chemical manipulation of glucose metabolism in porcine oocytes: effects on nuclear and cytoplasmic maturation
                    in vitro

Jason R Herrick; Amber M Brad; Rebecca L Krisher

doi:10.1530/rep.1.00835

journal article Feb 01, 2006

Chemical manipulation of glucose metabolism in porcine oocytes: effects on nuclear and cytoplasmic maturation in vitro

Jason R Herrick Amber M Brad Rebecca L Krisher

Reproduction Vol. 131 No. 2 pp. 289-298 · Oxford University Press (OUP)

View at Publisher Save 10.1530/rep.1.00835

Abstract

Abstract
The objectives of this study were to manipulate metabolism of glucose through glycolysis and the pentose phosphate pathway (PPP) in porcine oocytes during in vitro maturation, and determine the effects of this manipulation on meiotic progression, intracellular glutathione (GSX) concentrations and embryonic development. Cumulus-oocyte complexes isolated from abattoir ovaries were matured (40–44 h) in Purdue Porcine Medium for maturation alone (control) or supplemented with pyrroline-5 carboxylate (PC, 0.1 μM; PPP stimulator), diphenyleneiodonium (DPI, 0.1 μM; PPP inhibitor), dinitrophenol (DNP, 10 μM; glycolytic stimulator), hexametaphosphate (HMP, 100 μM; glycolytic inhibitor), PC + HMP or DNP + DPI. At the conclusion of in vitro maturation, cumulus cells were removed and oocytes were randomly allocated for analysis of GSX, metabolism and nuclear maturation, or in vitro fertilization and embryo culture. Both DPI and DNP + DPI decreased (P ≤ 0.05) the activity of glycolysis and the PPP, increased (P ≤ 0.05) the percentage of immature oocytes, and decreased (P ≤ 0.05) the proportion of mature oocytes compared with control oocytes and oocytes from the other treatments. Embryonic development (cleavage and blastocyst stage) and the intracellular content of GSX were also decreased (P ≤ 0.05) following exposure to DPI or DNP + DPI compared with control oocytes and oocytes from the other treatments. Oocyte metabolism, nuclear maturation, GSX content and embryonic development were unaffected (P &gt; 0.05) following exposure to PC, DNP, HMP or PC + HMP. Our results suggest that metabolism of glucose through the PPP and/or glycolysis plays a key role in the control of nuclear and cytoplasmic maturation of porcine oocytes in vitro.

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Details

Published: Feb 01, 2006
Vol/Issue: 131(2)
Pages: 289-298
License: View

Authors

J

Jason R Herrick

Department of Animal Sciences, Program of Comparative Medicine, Lilly Hall of Life Sciences, 915 West State Street, Purdue University, West Lafayette, Indiana 47907, USA

A

Amber M Brad

Department of Animal Sciences, Program of Comparative Medicine, Lilly Hall of Life Sciences, 915 West State Street, Purdue University, West Lafayette, Indiana 47907, USA

R

Rebecca L Krisher

Department of Animal Sciences, Program of Comparative Medicine, Lilly Hall of Life Sciences, 915 West State Street, Purdue University, West Lafayette, Indiana 47907, USA

Cite This Article

Jason R Herrick, Amber M Brad, Rebecca L Krisher (2006). Chemical manipulation of glucose metabolism in porcine oocytes: effects on nuclear and cytoplasmic maturation in vitro. Reproduction, 131(2), 289-298. https://doi.org/10.1530/rep.1.00835

Chemical manipulation of glucose metabolism in porcine oocytes: effects on nuclear and cytoplasmic maturation in vitro

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