Establishment of dsDNA/GNs/chit/GCE biosensor and electrochemical study on interaction between 6-mercaptopurine and DNA

Wenwei Tang; Min Zhang; Xinping Zeng

doi:10.3233/bme-130905

journal article Jan 01, 2014

Establishment of dsDNA/GNs/chit/GCE biosensor and electrochemical study on interaction between 6-mercaptopurine and DNA

Wenwei Tang Min Zhang

Xinping Zeng

Bio-Medical Materials and Engineering Vol. 24 No. 1 pp. 1071-1077 · SAGE Publications

View at Publisher Save 10.3233/bme-130905

Abstract

In this paper, the anti-cancer drug 6-mercaptopurine (6-MP) was taken as the detection object. The biosensor of dsDNA/GNs/chit/GCE was established using the grapheme (GNs) and chitosan (chit) as the compound modified material. The electrochemical behavior of 6-MP on the sensor was discussed, and the damage and its mechanism of 6-MP on DNA were studied. The experimental result showed that, after the modification of GNs-chit, the electrode activation area of GNs/chit/GCE increased remarkably, which was improved from 1.76cm
2
to 8.64 cm
2
, and the responsive oxidation peak current of GNs/chit/GCE to K
3
[Fe(CN)
6
] also increased remarkably. At the meantime, it was demonstrated that DNA was effectively fixed on the GNs/chit/GCE electrode;6-MP caused obvious damage to dsDNA, and the damage degree on the adenine was bigger than that on the guanine; the interaction between 6-MP and dsDNA was preliminarily deduced as the intercalation, and its electrochemical oxidation process was an irreversible process controlled by the adsorption.

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Details

Published: Jan 01, 2014
Vol/Issue: 24(1)
Pages: 1071-1077
License: View

Authors

W

Wenwei Tang

Department of Chemistry, Tongji University, Shanghai, 200092, China

M

Min Zhang

School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China

X

Xinping Zeng

School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China

Cite This Article

Wenwei Tang, Min Zhang, Xinping Zeng (2014). Establishment of dsDNA/GNs/chit/GCE biosensor and electrochemical study on interaction between 6-mercaptopurine and DNA. Bio-Medical Materials and Engineering, 24(1), 1071-1077. https://doi.org/10.3233/bme-130905

Establishment of dsDNA/GNs/chit/GCE biosensor and electrochemical study on interaction between 6-mercaptopurine and DNA

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