Host Starvation and Female Sex Influence Enterobacterial ClpB Production: A Possible Link to the Etiology of Eating Disorders

Jonathan Breton; Justine Jacquemot; Linda Yaker; Camille Leclerc; Nathalie Connil; Marc Feuilloley; Pierre Déchelotte; Sergueï O. Fetissov

doi:10.3390/microorganisms8040530

journal article Open Access Apr 07, 2020

Host Starvation and Female Sex Influence Enterobacterial ClpB Production: A Possible Link to the Etiology of Eating Disorders

Jonathan Breton Justine Jacquemot Linda Yaker Camille Leclerc Nathalie Connil Marc Feuilloley

Pierre Déchelotte

Sergueï O. Fetissov

Microorganisms Vol. 8 No. 4 pp. 530 · MDPI AG

View at Publisher Save 10.3390/microorganisms8040530

Abstract

Altered signaling between gut bacteria and their host has recently been implicated in the pathophysiology of eating disorders, whereas the enterobacterial caseinolytic protease B (ClpB) may play a key role as an antigen mimetic of α-melanocyte-stimulating hormone, an anorexigenic neuropeptide. Here, we studied whether ClpB production by gut bacteria can be modified by chronic food restriction and female sex, two major risk factors for the development of eating disorders. We found that food restriction increased ClpB DNA in feces and ClpB protein in plasma in both male and female rats, whereas females displayed elevated basal ClpB protein levels in the lower gut and plasma as well as increased ClpB-reactive immunoglobulins (Ig)M and IgG. In contrast, direct application of estradiol in E. coli cultures decreased ClpB concentrations in bacteria, while testosterone had no effect. Thus, these data support a mechanistic link between host-dependent risk factors of eating disorders and the enterobacterial ClpB protein production.

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Details

Published: Apr 07, 2020
Vol/Issue: 8(4)
Pages: 530
License: View

Authors

J

Jonathan Breton

Inserm UMR 1073, Nutrition, Gut and Brain Laboratory, 76183 Rouen, France; Institute for Research and innovation in Biomedicine, University of Rouen Normandy, 76000 Rouen, France; Rouen University Hospital, CHU Charles Nicolle, 76183 Rouen, France

J

Justine Jacquemot

Inserm UMR 1073, Nutrition, Gut and Brain Laboratory, 76183 Rouen, France; Institute for Research and innovation in Biomedicine, University of Rouen Normandy, 76000 Rouen, France

L

Linda Yaker

Inserm UMR 1073, Nutrition, Gut and Brain Laboratory, 76183 Rouen, France; Institute for Research and innovation in Biomedicine, University of Rouen Normandy, 76000 Rouen, France

C

Camille Leclerc

Institute for Research and innovation in Biomedicine, University of Rouen Normandy, 76000 Rouen, France; Laboratory of Microbiology Signals and Microenvironment, LMSM EA4312, F-27000 Evreux, France

N

Nathalie Connil

Institute for Research and innovation in Biomedicine, University of Rouen Normandy, 76000 Rouen, France; Laboratory of Microbiology Signals and Microenvironment, LMSM EA4312, F-27000 Evreux, France

M

Marc Feuilloley

Institute for Research and innovation in Biomedicine, University of Rouen Normandy, 76000 Rouen, France; Laboratory of Microbiology Signals and Microenvironment, LMSM EA4312, F-27000 Evreux, France

P

Pierre Déchelotte

Inserm UMR 1073, Nutrition, Gut and Brain Laboratory, 76183 Rouen, France; Institute for Research and innovation in Biomedicine, University of Rouen Normandy, 76000 Rouen, France; Rouen University Hospital, CHU Charles Nicolle, 76183 Rouen, France

S

Sergueï O. Fetissov

Inserm UMR 1073, Nutrition, Gut and Brain Laboratory, 76183 Rouen, France; Institute for Research and innovation in Biomedicine, University of Rouen Normandy, 76000 Rouen, France; Inserm UMR1239, Neuronal and Neuroendocrine Differentiation and Communication Laboratory, 76130 Mont-Saint-Aignan, France

Cite This Article

Jonathan Breton, Justine Jacquemot, Linda Yaker, et al. (2020). Host Starvation and Female Sex Influence Enterobacterial ClpB Production: A Possible Link to the Etiology of Eating Disorders. Microorganisms, 8(4), 530. https://doi.org/10.3390/microorganisms8040530

Host Starvation and Female Sex Influence Enterobacterial ClpB Production: A Possible Link to the Etiology of Eating Disorders

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