Antimicrobial Photodynamic Inactivation Mediated by Rose Bengal and Erythrosine Is Effective in the Control of Food-Related Bacteria in Planktonic and Biofilm States

Alex Fiori Silva; Anabela Borges; Camila Fabiano Freitas; Noboru Hioka; Jane Martha Graton Mikcha; Manuel Simões

doi:10.3390/molecules23092288

journal article Open Access Sep 07, 2018

Antimicrobial Photodynamic Inactivation Mediated by Rose Bengal and Erythrosine Is Effective in the Control of Food-Related Bacteria in Planktonic and Biofilm States

Alex Fiori Silva

Anabela Borges

Camila Fabiano Freitas

Noboru Hioka

Jane Martha Graton Mikcha

Manuel Simões

Molecules Vol. 23 No. 9 pp. 2288 · MDPI AG

View at Publisher Save 10.3390/molecules23092288

Abstract

The thermal and chemical-based methods applied for microbial control in the food industry are not always environmentally friendly and may change the nutritional and organoleptic characteristics of the final products. Moreover, the efficacy of sanitizing agents may be reduced when microbial cells are enclosed in biofilms. The objective of this study was to investigate the effect of photodynamic inactivation, using two xanthene dyes (rose bengal and erythrosine) as photosensitizing agents and green LED as a light source, against Staphylococcus aureus, Listeria innocua, Enterococcus hirae and Escherichia coli in both planktonic and biofilm states. Both photosensitizing agents were able to control planktonic cells of all bacteria tested. The treatments altered the physicochemical properties of cells surface and also induced potassium leakage, indicating damage of cell membranes. Although higher concentrations of the photosensitizing agents (ranging from 0.01 to 50.0 μmol/L) were needed to be applied, the culturability of biofilm cells was reduced to undetectable levels. This finding was confirmed by the live/dead staining, where propidium iodide-labeled bacteria numbers reached up to 100%. The overall results demonstrated that photoinactivation by rose bengal and erythrosine may be a powerful candidate for the control of planktonic cells and biofilms in the food sector.

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Details

Published: Sep 07, 2018
Vol/Issue: 23(9)
Pages: 2288
License: View

Authors

A

Alex Fiori Silva

Postgraduate Program of Health Sciences, State University of Maringá, Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil; LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal

A

Anabela Borges

LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal

C

Camila Fabiano Freitas

Department of Chemistry, State University of Maringa, Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil

N

Noboru Hioka

Department of Chemistry, State University of Maringa, Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil

J

Jane Martha Graton Mikcha

Postgraduate Program of Health Sciences, State University of Maringá, Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil

M

Manuel Simões

LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal

Funding

Fundação para a Ciência e a Tecnologia Award: POCI-01-0145-FEDER-030219

Cite This Article

Alex Fiori Silva, Anabela Borges, Camila Fabiano Freitas, et al. (2018). Antimicrobial Photodynamic Inactivation Mediated by Rose Bengal and Erythrosine Is Effective in the Control of Food-Related Bacteria in Planktonic and Biofilm States. Molecules, 23(9), 2288. https://doi.org/10.3390/molecules23092288

Antimicrobial Photodynamic Inactivation Mediated by Rose Bengal and Erythrosine Is Effective in the Control of Food-Related Bacteria in Planktonic and Biofilm States

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