Cellulose-Chitosan-Nanohydroxyapatite Hybrid Composites by One-Pot Synthesis for Biomedical Applications

Katia Jarquin-Yáñez; Efrain Rubio-Rosas; Gabriela Piñón-Zárate; Andrés Castell-Rodríguez; Martha Poisot

doi:10.3390/polym13101655

journal article Open Access May 19, 2021

Cellulose-Chitosan-Nanohydroxyapatite Hybrid Composites by One-Pot Synthesis for Biomedical Applications

Katia Jarquin-Yáñez

Efrain Rubio-Rosas

Gabriela Piñón-Zárate Andrés Castell-Rodríguez

Martha Poisot

Polymers Vol. 13 No. 10 pp. 1655 · MDPI AG

View at Publisher Save 10.3390/polym13101655

Abstract

The development of organic–inorganic hybrid materials deserves special interest for bone tissue engineering applications, where materials must have properties that induce the survival and activation of cells derived from the mesenchyme. In this work, four bio-nanocomposites based on cellulose and variable content of chitosan, from 15 to 50 w% based on cellulose, with nanohydroxyapatite and β-Glycerophosphate as cross-linking agent were synthesized by simplified and low-energy-demanding solvent exchange method to determine the best ratio of chitosan to cellulose matrix. This study analyzes the metabolic activity and survival of human dermal fibroblast cells cultivated in four bio-nanocomposites based on cellulose and the variable content of chitosan. The biocompatibility was tested by the in vitro cytotoxicity assays Live/Dead and PrestoBlue. In addition, the composites were characterized by FTIR, XRD and SEM. The results have shown that the vibration bands of β-Glycerophosphate have prevailed over the other components bands, while new diffraction planes have emerged from the interaction between the cross-linking agent and the biopolymers. The bio-nanocomposite micrographs have shown no surface porosity as purposely designed. On the other hand, cell death and detachment were observed when the composites of 1 and 0.1 w/v% were used. However, the composite containing 10 w% chitosan, against the sum of cellulose and β-Glycerophosphate, has shown less cell death and detachment when used at 0.01 w/v%, making it suitable for more in vitro studies in bone tissue engineering, as a promising economical biomaterial.

Topics

No keywords indexed for this article. Browse by subject →

References

35

[1]

Bionanocomposites: A New Concept of Ecological, Bioinspired, and Functional Hybrid Materials

M. Darder, P. Aranda, E. Ruiz‐Hitzky

Advanced Materials 2007 10.1002/adma.200602328

[2]

Catauro "Investigation of bioactivity, biocompatibility and thermal behavior of sol–gel silica glass containing a high PEG percentage" Mater. Sci. Eng. C Mater Biol. Appl. (2016) 10.1016/j.msec.2015.11.077

[3]

Aliramaji "Super-paramagnetic responsive Silk Fibroin/chitosan/magnetite scaffolds with tunable pore structures for bone tissue engineering applications" Mater. Sci. Eng. C Mater. Biol. Appl. (2017) 10.1016/j.msec.2016.09.039

[4]

"Electrospinning production of PVA/CS/HEMA/nHA bionanocomposite" Int. J. Nano Biomater. (2019) 10.1504/ijnbm.2019.101754

[5]

Ali "Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites" Technol. Health Care (2020) 10.3233/thc-181568

[6]

Husain, S., Al-Samadani, K.H., Najeeb, S., Zafar, M.S., Khurshid, Z., Zohaib, S., and Qasim, S.B. (2017). Chitosan biomaterials for current and potential dental applications. Materials, 10. 10.3390/ma10060602

[7]

She "Preparation and in vitro degradation of porous three-dimensional silk fibroin/chitosan scaffold" Polym. Degrad. Stab. (2008) 10.1016/j.polymdegradstab.2008.04.001

[8]

Tavakol "In vitro and in vivo investigations on bone regeneration potential of laminated hydroxyapatite/gelatin nanocomposite scaffold along with DBM" J. Nanopart. Res. (2012) 10.1007/s11051-012-1265-y

[9]

Sionkowska "Characterization of collagen/hydroxyapatite composite sponges as a potential bone substitute" Int. J. Biol. Macromol. (2010) 10.1016/j.ijbiomac.2010.07.002

[10]

Tavakol "The effect of carrier type on bone regeneration of demineralized bone matrix in vivo" J. Craniofac. Surg. (2013) 10.1097/scs.0b013e3182a243d4

[11]

Zhao "Magnetic bioinspired micro/nanostructured composite scaffold for bone regeneration" Colloid Surface B (2019) 10.1016/j.colsurfb.2018.11.003

[12]

Tavakol "Investigating the effects of particle size and chemical structure on cytotoxicity and bacteriostatic potential of nano hydroxyapatite/chitosan/silica and nano hydroxyapatite/chitosan/silver; as antibacterial bone substitutes" J. Nanopart. Res (2014) 10.1007/s11051-014-2622-9

[13]

Gholizadeh "Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering" Int. J. Biol. Macromol. (2017) 10.1016/j.ijbiomac.2016.12.086

[14]

Hua "Transition from Bioinert to Bioactive Material by Tailoring the Biological Cell Response to Carboxylated Nanocellulose" Biomacromolecules (2016) 10.1021/acs.biomac.6b00053

[15]

Capadona "A versatile approach for the processing of polymer nanocomposites with self-assambled nanofibre templates" Nat. Nanotechnol. (2007) 10.1038/nnano.2007.379

[16]

Eichhorn "Current international research into cellulose nanofibres and nanocomposites" J. Mater. Sci. (2010) 10.1007/s10853-009-3874-0

[17]

Rodríguez-Robledo, M.C., González-Lozano, M.A., Ponce-Peña, P., Quintana Owen, P., Aguilar-González, M.A., Nieto-Castañeda, G., Bazán-Mora, E., López-Martínez, R., Ramírez-Galicia, G., and Poisot, M. (2018). Cellulose-Silica Nanocomposites of High Reinforcing Content with Fungi Decay Resistance by One-Pot Synthesis. Materials, 11. 10.3390/ma11040575

[18]

Chen "The photoluminescence, drug delivery and imaging properties of multifunctional Eu3+/Gd3+ dual-doped hydroxyapatite nanorods" Biomaterials (2011) 10.1016/j.biomaterials.2011.08.032

[19]

Lak "Rapid Formation of Mono-Dispersed Hydroxyapatite Nanorods with Narrow-Size Distribution via Microwave Irradiation" J. Am. Ceram. Soc. (2008) 10.1111/j.1551-2916.2008.02690.x

[20]

Delgado Jiménez, J.F. (2013). Síntesis de Nanopartículas de Hidroxiapatita Dopada con Eu3+ por Irradiación de Microondas. [Bacherol’s Thesis, Universidad de las Americas Puebla].

[21]

Skwarczynska "The structural (FTIR, XRD, and XPS) and biological studies of thermosensitive chitosan chloride gels with β-glycerophosphate disodium" J. Appl. Polym. Sci. (2018) 10.1002/app.46459

[22]

Tabaght "Cellulose grafted aliphatic polyesters: Synthesis, characterization and biodegradation under controlled conditions in a laboratory test system" J. Mol. Struct. (2020) 10.1016/j.molstruc.2019.127582

[23]

Lin "Quantitative analyses of the effect of silk fibroin/nano-hydroxyapatite composites on osteogenic differentiation of MG-63 human osteosarcoma cells" J. Biosci. Bioeng. (2015) 10.1016/j.jbiosc.2014.10.009

[24]

Pascu "Electrospun composites of PHBV, silk fibroin and nano-hydroxyapatite for bone tissue engineering" Mater. Sci. Eng. C Mater Biol. Appl. (2013) 10.1016/j.msec.2013.08.012

[25]

Mobika "Substantial effect of Silk fibroin reinforcement on properties of Hydroxyapatite/Silk fibroin nanocomposite for bone tissue engineering application" J. Mol. Struct. (2020) 10.1016/j.molstruc.2020.127739

[26]

Fazel, R. (2011). Hydroxyapatite-Based Materials: Synthesis and Characterization, Biomedical Engineering—Frontiers and Challenges, InTech.

[27]

Pereira "Performance of chitosan/glycerol phosphate hydrogel as a support for lipase immobilization" Mat. Res. (2017) 10.1590/1980-5373-mr-2017-0091

[28]

The Powder Diffraction File: a quality materials characterization database

Stacy Gates-Rector, Thomas Blanton

Powder Diffraction 2019 10.1017/s0885715619000812

[29]

Idealized powder diffraction patterns for cellulose polymorphs

Alfred D. French

Cellulose 2014 10.1007/s10570-013-0030-4

[30]

Fawcett "Reference materials for the study of polymorphism and crystallinity in cellulosics" Powder Diffr. (2013) 10.1017/s0885715612000930

[31]

Hammond, C. (1998). The Basics of Crystallography and Diffraction, IUCr Texts on Crystallography, No 3, Oxford University Press.

[32]

Niranjan "A novel injectable temperature-sensitive zinc doped chitosan/β-glycerophosphate hydrogel for bone tissue engineering" Int. J. Biol. Macromol. (2013) 10.1016/j.ijbiomac.2012.11.026

[33]

Nazzal "Ex-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrix" Sci. Rep. (2020) 10.1038/s41598-020-78477-x

[34]

Pfeffer "Streamlined duplex live-dead microplate assay for cultured cells" Exp. Eye Res. (2017) 10.1016/j.exer.2017.05.011

[35]

Ayyagari, V.N., Diaz-Sylvester, P.L., Jeff Hsieh, T.H., and Brard, L. (2017). Evaluation of the cytotoxicity of the Bithionol-paclitaxel combination in a panel of human ovarian cancer cell lines. PLoS ONE, 12. 10.1371/journal.pone.0185111

Cited By

5

Hydroxyapatite–cellulose composites: properties, fabrication methods, and applications

Soumia Berrahou, Souhayla Latifi · 2026

Journal of Materials Science: Mater...

Metrics

5

Citations

35

References

Details

Published: May 19, 2021
Vol/Issue: 13(10)
Pages: 1655
License: View

Authors

K

Katia Jarquin-Yáñez

Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Interior, Ciudad Universitaria, CdMx, C.P. 04510 Coyoacan, Mexico

E

Efrain Rubio-Rosas

Dirección de Innovación y Transferencia de Tecnología, Benemérita Universidad Autónoma de Puebla, Prolongación de la 24 Sur y Av. San Claudio, Ciudad Universitaria, Col. San Manuel, C.P. 72570 Puebla, Mexico

G

Gabriela Piñón-Zárate

Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Interior, Ciudad Universitaria, CdMx, C.P. 04510 Coyoacan, Mexico

A

Andrés Castell-Rodríguez

Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Circuito Interior, Ciudad Universitaria, CdMx, C.P. 04510 Coyoacan, Mexico

M

Martha Poisot

Universidad del Papaloapan, Instituto de Química Aplicada, Circuito Central 200, Parque Industrial, C.P. 68301 Tuxtepec, Mexico

Funding

Universidad Nacional Autónoma de México Award: PAPIIT IA207917

Cite This Article

Katia Jarquin-Yáñez, Efrain Rubio-Rosas, Gabriela Piñón-Zárate, et al. (2021). Cellulose-Chitosan-Nanohydroxyapatite Hybrid Composites by One-Pot Synthesis for Biomedical Applications. Polymers, 13(10), 1655. https://doi.org/10.3390/polym13101655

Cellulose-Chitosan-Nanohydroxyapatite Hybrid Composites by One-Pot Synthesis for Biomedical Applications

You May Also Like