Three‐dimensional bioprinting of stem cell‐derived central nervous system cells enables astrocyte growth, vasculogenesis, and enhances neural differentiation/function

Michael A. Sullivan; Samuel Lane; Alexander Volkerling; Martin Engel; Eryn L. Werry; Michael Kassiou

doi:10.1002/bit.28470

journal article Open Access Jul 03, 2023

Three‐dimensional bioprinting of stem cell‐derived central nervous system cells enables astrocyte growth, vasculogenesis, and enhances neural differentiation/function

Michael A. Sullivan Samuel Lane Alexander Volkerling Martin Engel Eryn L. Werry

Michael Kassiou

Biotechnology and Bioengineering Vol. 120 No. 10 pp. 3079-3091 · Wiley

View at Publisher Save 10.1002/bit.28470

Abstract

AbstractCurrent research tools for preclinical drug development such as rodent models and two‐dimensional immortalized monocultures have failed to serve as effective translational models for human central nervous system (CNS) disorders. Recent advancements in the development of induced pluripotent stem cells (iPSCs) and three‐dimensional (3D) culturing can improve the in vivo‐relevance of preclinical models, while generating 3D cultures though novel bioprinting technologies can offer increased scalability and replicability. As such, there is a need to develop platforms that combine iPSC‐derived cells with 3D bioprinting to produce scalable, tunable, and biomimetic cultures for preclinical drug discovery applications. We report a biocompatible poly(ethylene glycol)‐based matrix which incorporates Arg‐Gly‐Asp and Tyr‐Ile‐Gly‐Ser‐Arg peptide motifs and full‐length collagen IV at a stiffness similar to the human brain (1.5 kPa). Using a high‐throughput commercial bioprinter we report the viable culture and morphological development of monocultured iPSC‐derived astrocytes, brain microvascular endothelial‐like cells, neural progenitors, and neurons in our novel matrix. We also show that this system supports endothelial‐like vasculogenesis and enhances neural differentiation and spontaneous activity. This platform forms a foundation for more complex, multicellular models to facilitate high‐throughput translational drug discovery for CNS disorders.

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Details

Published: Jul 03, 2023
Vol/Issue: 120(10)
Pages: 3079-3091
License: View

Authors

M

Michael A. Sullivan

School of Medical Sciences, The Faculty of Medicine and Health The University of Sydney Sydney New South Wales Australia

S

Samuel Lane

School of Chemistry, The Faculty of Science The University of Sydney Sydney New South Wales Australia

A

Alexander Volkerling

Inventia Life Science Operations Pty Ltd. Alexandria New South Wales Australia

M

Martin Engel

Inventia Life Science Operations Pty Ltd. Alexandria New South Wales Australia

E

Eryn L. Werry

School of Chemistry, The Faculty of Science The University of Sydney Sydney New South Wales Australia; Central Clinical School, Faculty of Medicine and Health The University of Sydney Sydney New South Wales Australia

M

Michael Kassiou

School of Chemistry, The Faculty of Science The University of Sydney Sydney New South Wales Australia

Funding

National Health and Medical Research Council

Cite This Article

Michael A. Sullivan, Samuel Lane, Alexander Volkerling, et al. (2023). Three‐dimensional bioprinting of stem cell‐derived central nervous system cells enables astrocyte growth, vasculogenesis, and enhances neural differentiation/function. Biotechnology and Bioengineering, 120(10), 3079-3091. https://doi.org/10.1002/bit.28470

Three‐dimensional bioprinting of stem cell‐derived central nervous system cells enables astrocyte growth, vasculogenesis, and enhances neural differentiation/function

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