Effect of auxin and phenobarbital on the ultrastructure and digitoxin content inDigitalis purpureatissue culture

Mercedes Bonfill; Javier Palazon; Rosa M. Cusidó; M. Teresa Piñol; Carmen Morales

doi:10.1139/b96-047

journal article Mar 01, 1996

Effect of auxin and phenobarbital on the ultrastructure and digitoxin content inDigitalis purpureatissue culture

Mercedes Bonfill Javier Palazon

Rosa M. Cusidó M. Teresa Piñol Carmen Morales

Canadian Journal of Botany Vol. 74 No. 3 pp. 378-382 · Canadian Science Publishing

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Abstract

Callus derived from Digitalis purpurea hypocotils were grown during a 6-week period on solid Murashige–Skoog medium supplemented with 1 mg/L 6-benzylaminopurine, 0.01 mg/L gibberellic acid and 0.1 mg/L indole-3-acetic acid or α-naphthaleneacetic acid, with or without phenobarbital (40 mg/L). The presence of phenobarbital in the culture medium caused a reduction of the vacuole/cytoplasm ratio. At the same time, the chloroplastic volume fraction decreased in callus tissue cells grown in media supplemented with phenobarbital, while the mitochondrial volume ratio increased. Digitoxin content was enhanced in callus tissues, especially in those grown on indole-3-acetic acid medium supplemented with phenobarbital. The relationship between ultrastructure of D. purpurea callus and digitoxin content is discussed. Keywords: Digitalis purpurea tissue cultures, digitoxin, phenobarbital, mitochondria, chloroplast.

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Published: Mar 01, 1996
Vol/Issue: 74(3)
Pages: 378-382
License: View

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Cite This Article

Mercedes Bonfill, Javier Palazon, Rosa M. Cusidó, et al. (1996). Effect of auxin and phenobarbital on the ultrastructure and digitoxin content inDigitalis purpureatissue culture. Canadian Journal of Botany, 74(3), 378-382. https://doi.org/10.1139/b96-047

Effect of auxin and phenobarbital on the ultrastructure and digitoxin content inDigitalis purpureatissue culture

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