Apical constriction is driven by a pulsatile apical myosin network in delaminating Drosophila neuroblasts

Yanru An; Guosheng Xue; Yang Shaobo; Deng Mingxi; Xiaowei Zhou; Weichuan Yu; Toyotaka Ishibashi; Lei Zhang; Yan Yan

doi:10.1242/dev.150763

journal article Jan 01, 2017

Apical constriction is driven by a pulsatile apical myosin network in delaminating Drosophila neuroblasts

Yanru An Guosheng Xue Yang Shaobo Deng Mingxi Xiaowei Zhou

Weichuan Yu Toyotaka Ishibashi

Lei Zhang

Yan Yan

Development · The Company of Biologists

View at Publisher Save 10.1242/dev.150763

Abstract

Cell delamination is a conserved morphogenetic process important for generation of cell diversity and maintenance of tissue homeostasis. Here we used Drosophila embryonic neuroblasts as a model to study the apical constriction process during cell delamination. We observe dynamic myosin signals both around the cell adherens junctions and underneath the cell apical surface in the neuroectoderm. On the cell apical cortex the non-junctional myosin forms flows and pulses, which are termed as medial myosin pulses. Quantitative differences in medial myosin pulse intensity and frequency are critical to distinguish delaminating neuroblasts from their neighbors. Inhibition of medial myosin pulses blocks delamination. The fate of neuroblasts is set apart from their neighbors by Notch signaling-mediated lateral inhibition. When we inhibit Notch signaling activity in the embryo, we observe that small clusters of cells undergo apical constriction and display an abnormal apical myosin pattern. Together, we demonstrate that a contractile actomyosin network across the apical cell surface is organized to drive apical constriction in delaminating neuroblasts.

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Metrics

39

Citations

45

References

Details

Published: Jan 01, 2017
License: View

Authors

Y

Yanru An

Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

G

Guosheng Xue

Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

Y

Yang Shaobo

Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

D

Deng Mingxi

Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

X

Xiaowei Zhou

Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

W

Weichuan Yu

Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

T

Toyotaka Ishibashi

Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

L

Lei Zhang

Beijing International Center for Mathematical Research, Peking University, Beijing, China; Center for Quantitative Biology, Peking University, Beijing, China

Y

Yan Yan

Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

Funding

National Natural Science Foundation of China Award: 11622102

University Grants Committee Award: GRF16103815

Cite This Article

Yanru An, Guosheng Xue, Yang Shaobo, et al. (2017). Apical constriction is driven by a pulsatile apical myosin network in delaminating Drosophila neuroblasts. Development. https://doi.org/10.1242/dev.150763

Apical constriction is driven by a pulsatile apical myosin network in delaminating Drosophila neuroblasts

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