RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi

Isabel Padilla-Roji; Laura Ruiz-Jiménez; Nisrine Bakhat; Alejandra Vielba-Fernández; Alejandro Pérez-García; Dolores Fernández-Ortuño

doi:10.3390/ijms24109082

journal article Open Access May 22, 2023

RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi

Isabel Padilla-Roji Laura Ruiz-Jiménez

Nisrine Bakhat

Alejandra Vielba-Fernández Alejandro Pérez-García

Dolores Fernández-Ortuño

International Journal of Molecular Sciences Vol. 24 No. 10 pp. 9082 · MDPI AG

View at Publisher Save 10.3390/ijms24109082

Abstract

Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determined by the presence of haustoria, specialized fungal cells that are responsible for nutrient uptake and molecular dialogue with the host, a fact that undoubtedly complicates their study under laboratory conditions, especially in terms of genetic manipulation. RNA interference (RNAi) is the biological process of suppressing the expression of a target gene through double-stranded RNA that induces mRNA degradation. RNAi technology has revolutionized the study of these obligate biotrophic fungi by enabling the analysis of gene function in these fungal. More importantly, RNAi technology has opened new perspectives for the management of powdery mildew and rust diseases, first through the stable expression of RNAi constructs in transgenic plants and, more recently, through the non-transgenic approach called spray-induced gene silencing (SIGS). In this review, the impact of RNAi technology on the research and management of powdery mildew and rust fungi will be addressed.

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Details

Published: May 22, 2023
Vol/Issue: 24(10)
Pages: 9082
License: View

Authors

I

Isabel Padilla-Roji