Synthetic Microbial Community Isolated from Intercropping System Enhances P Uptake in Rice

Huimin Ma; Hongcheng Zhang; Congcong Zheng; Zonghui Liu; Jing Wang; Ping Tian; Zhihai Wu; Hualiang Zhang

doi:10.3390/ijms252312819

journal article Open Access Nov 28, 2024

Synthetic Microbial Community Isolated from Intercropping System Enhances P Uptake in Rice

Huimin Ma

Hongcheng Zhang

Congcong Zheng

Zonghui Liu Jing Wang

Ping Tian

Zhihai Wu Hualiang Zhang

International Journal of Molecular Sciences Vol. 25 No. 23 pp. 12819 · MDPI AG

View at Publisher Save 10.3390/ijms252312819

Abstract

Changes in root traits and rhizosphere microbiome are important ways to optimize plant phosphorus (P) efficiency and promote multifunctionality in intercropping. However, whether and how synthetic microbial communities isolated from polyculture systems can facilitate plant growth and P uptake are still largely unknown. A field experiment was first carried out to assess the rice yield and P uptake in the rice/soybean intercropping systems, and a synthetic microbial community (SynCom) isolated from intercropped rice was then constructed to elucidate the potential mechanisms of growth-promoting effects on rice growth and P uptake in a series of pot experiments. Our results showed that the yield and P uptake of intercropped rice were lower than those of rice grown in monoculture. However, bacterial networks in the rice rhizosphere were more stable in polyculture, exhibiting more hub nodes and greater modularity compared to the rice monoculture. A bacterial synthetic community (SynCom) composed of four bacterial strains (Variovorax paradoxus, Novosphingobium subterraneum, Hydrogenophaga pseudoflava, Acidovorax sp.) significantly enhanced the biomass and P uptake of potted rice plants. These growth-promoting effects are underpinned by multiple pathways, including the direct activation of soil available P, increased root surface area and root tip number, reduced root diameter, and promotion of root-to-shoot P translocation through up-regulation of Pi transporter genes (OsPht1;1, OsPht1;2, OsPht1;4, OsPht1;6). This study highlights the potential of harnessing synthetic microbial communities to enhance nutrient acquisition and improve crop production.

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Published: Nov 28, 2024
Vol/Issue: 25(23)
Pages: 12819
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Huimin Ma